Control of Enzyme Reactivity in Response to Osmotic Pressure Modulation Mimicking Dynamic Assembly of Intracellular Organelles.
| dc.rights.license | open | en_US |
| hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
| hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
| dc.contributor.author | SCHVARTZMAN, Clémence | |
| hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
| hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
| dc.contributor.author | ZHAO, Hang | |
| hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
| hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
| dc.contributor.author | IBARBOURE, Emmanuel | |
| hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
| hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
| dc.contributor.author | IBRAHIMOVA, Vusala | |
| hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
| hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
| dc.contributor.author | GARANGER, Elisabeth
IDREF: 089451740 | |
| hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
| hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
| dc.contributor.author | LECOMMANDOUX, Sébastien | |
| dc.date.accessioned | 2023-05-29T17:00:53Z | |
| dc.date.available | 2023-05-29T17:00:53Z | |
| dc.date.issued | 2023-05-07 | |
| dc.identifier.issn | 1521-4095 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/182352 | |
| dc.description.abstractEn | In response to variations in osmotic stress, in particular to hypertonicity associated with biological dysregulations, cells have developed complex mechanisms to release their excess water, thus avoiding their bursting and death. When water is expelled, cells shrink and concentrate their internal bio(macro)molecular content, inducing the formation of membraneless organelles following a liquid-liquid phase separation (LLPS) mechanism. To mimic this intrinsic property of cells, functional thermo-responsive elastin-like polypeptide (ELP) biomacromolecular conjugates are herein encapsulated into self-assembled lipid vesicles using a microfluidic system, together with polyethylene glycol (PEG) to mimic cells' interior crowded microenvironment. By inducing a hypertonic shock onto the vesicles, expelled water induces a local increase of concentration and a concomitant decrease of the cloud point temperature (T ) of ELP bioconjugates that phase separate and form coacervates mimicking cellular stress-induced membraneless organelle assemblies. Horseradish peroxidase (HRP), as a model enzyme, is bioconjugated to ELPs and is locally confined in coacervates as a response to osmotic stress. This consequently increases local HRP and substrate concentrations and accelerates the kinetics of the enzymatic reaction. These results illustrate a unique way to fine-tune enzymatic reactions dynamically as a response to a physiological change in isothermal conditions. This article is protected by copyright. All rights reserved. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution-NonCommercial-ShareAlike 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/us/ | * |
| dc.title.en | Control of Enzyme Reactivity in Response to Osmotic Pressure Modulation Mimicking Dynamic Assembly of Intracellular Organelles. | |
| dc.title.alternative | Adv Mater | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1002/adma.202301856 | en_US |
| dc.subject.hal | Chimie/Polymères | en_US |
| dc.identifier.pubmed | 37149761 | en_US |
| bordeaux.journal | Advanced Materials | en_US |
| bordeaux.page | e2301856 | en_US |
| bordeaux.volume | 35 | en_US |
| bordeaux.hal.laboratories | Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | Bordeaux INP | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-04109203 | |
| hal.version | 1 | |
| hal.date.transferred | 2023-05-29T17:00:57Z | |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | CC BY-NC-SA | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Advanced%20Materials&rft.date=2023-05-07&rft.volume=35&rft.spage=e2301856&rft.epage=e2301856&rft.eissn=1521-4095&rft.issn=1521-4095&rft.au=SCHVARTZMAN,%20Cl%C3%A9mence&ZHAO,%20Hang&IBARBOURE,%20Emmanuel&IBRAHIMOVA,%20Vusala&GARANGER,%20Elisabeth&rft.genre=article |
