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Cassette recruitment in the chromosomal Integron of Vibrio cholerae

dc.rights.licenseopenen_US
dc.contributor.authorVIT, Claire
dc.contributor.authorRICHARD, Egill
dc.contributor.authorFOURNES, Florian
dc.contributor.authorWHITEWAY, Clémence
dc.contributor.authorEYER, Xavier
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLAPAILLERIE, Delphine
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPARISSI, Vincent
dc.contributor.authorMAZEL, Didier
dc.contributor.authorLOOT, Céline
dc.date.accessioned2023-05-22T14:20:52Z
dc.date.available2023-05-22T14:20:52Z
dc.date.issued2021-06-04
dc.identifier.issn0305-1048en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/182238
dc.description.abstractEnIntegrons are genetic systems conferring to bacteria a rapid adaptation capability. The integron integrase is able to capture, stockpile and shuffle novel functions embedded in cassettes. This involves the recognition of both substrates, the attI site, and the cassette associated attC sites. Integrons can be sedentary and chromosomally located (SCI) or, carried by conjugative plasmids (Mobile Integron, MI), hence favoring their dissemination among bacteria. Here, for the first time, we investigate the cassette recruitment in the Vibrio cholerae SCI during conjugation and natural transformation. We demonstrated that horizontally transferred cassette can be recruited inside the chromosomal integron. The endogenous integrase expression is sufficiently triggered, after SOS response induction mediated by the entry of single-stranded cassettes during conjugation and natural transformation, to mediate significant cassette insertion. We demonstrate that the attIA insertion is preferential, despite the presence of 180 attC sites in the integron array. Thanks to the presence of a promoter in the attIA site vicinity, all these newly inserted cassettes are expressed and prone to adaptive selection. We also show that the RecA protein is critical for cassette recruitment in V. cholerae SCI but not in MIs. Moreover, a contrario to MIs, the V. cholerae SCI is not active in others bacterial hosts. MIs might have evolved from the SCIs by overcoming host factors, which would explain their large dissemination in bacteria and their role in the antibioresistance expansion.
dc.description.sponsorshipIntegrative Biology of Emerging Infectious Diseases - ANR-10-LABX-0062en_US
dc.language.isoENen_US
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.meshChromosomes
dc.subject.meshEscherichia coli
dc.subject.meshGene Expression Regulation, Bacterial
dc.subject.meshGene Transfer, Horizontal
dc.subject.meshIntegrases
dc.subject.meshIntegrons
dc.subject.meshRec A Recombinases
dc.subject.meshRecombination, Genetic
dc.subject.meshVibrio cholerae
dc.title.enCassette recruitment in the chromosomal Integron of Vibrio cholerae
dc.typeArticle de revueen_US
dc.identifier.doi10.1093/nar/gkab412en_US
dc.subject.halSciences du Vivant [q-bio]en_US
dc.subject.halSciences du Vivant [q-bio]/Biodiversité/Evolution [q-bio.PE]en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologieen_US
bordeaux.journalNucleic Acids Researchen_US
bordeaux.page5654-5670en_US
bordeaux.volume49en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue10en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-03084487
hal.version1
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nucleic%20Acids%20Research&rft.date=2021-06-04&rft.volume=49&rft.issue=10&rft.spage=5654-5670&rft.epage=5654-5670&rft.eissn=0305-1048&rft.issn=0305-1048&rft.au=VIT,%20Claire&RICHARD,%20Egill&FOURNES,%20Florian&WHITEWAY,%20Cl%C3%A9mence&EYER,%20Xavier&rft.genre=article


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