Using Optical Lattice for STED Parallelization
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en
Communication dans un congrès avec actes
Este ítem está publicado en
Nanoimaging and Nanospectroscopy II, SPIE Optics+Photonics, 2014-08-17, San Diego. 2014-08-18p. 91690E
Resumen en inglés
Being a scanning microscopy, Stimulated Emission Depletion (STED) needs to be parallelized for fast wide-field imaging. Here, we achieve large parallelization of STED microscopy using well-designed Optical Lattice (OL) for ...Leer más >
Being a scanning microscopy, Stimulated Emission Depletion (STED) needs to be parallelized for fast wide-field imaging. Here, we achieve large parallelization of STED microscopy using well-designed Optical Lattice (OL) for depletion, together with a fast camera for detection. Depletion optical lattices with 100 intensity "zeros" are generated by four-beam interference. Scanning only a unit cell, as small as 290 nm by 290 nm, of the depletion OL is sufficient for STED imaging. The OL-STED microscopy acquires super-resolution images with 70 nm resolution and at a rate of 12.5 frames per image.< Leer menos
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