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hal.structure.identifierlp2n-04,lp2n-12
dc.contributor.authorLEDUC, Cecile
hal.structure.identifierDepartment of Computer Science [Dresden]
dc.contributor.authorPADBERG-GEHLE, Kathrin
hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorVARGA, Vladimir
hal.structure.identifierChair of Sociology, in particular of Modeling and Simulation [SOMS]
dc.contributor.authorHELBING, Dirk
hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorDIEZ, Stefan
hal.structure.identifierMax Planck Institute of Molecular Cell Biology and Genetics [MPI-CBG]
dc.contributor.authorHOWARD, Jonathon
dc.date.accessioned2023-05-12T10:24:56Z
dc.date.available2023-05-12T10:24:56Z
dc.date.conference2012-03-25
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181229
dc.description.abstractEnDespite the crowdedness of the interior of cells, microtubule-based motor proteins are able to deliver cargoes rapidly and reliably throughout the cytoplasm. We hypothesize that motor proteins may be adapted to operate in crowded environments by having molecular properties that prevent them from forming traffic jams. To test this hypothesis, we reconstituted high-density traffic of purified kinesin-8 motor protein, a highly processive motor with long end-residency time, along microtubules in a total internal-reflection fluorescence microscopy assay. We found that traffic jams, characterized by an abrupt increase in the density of motors with an associated abrupt decrease in motor speed, form even in the absence of other obstructing proteins. To determine the molecular properties that lead to jamming, we altered the concentration of motors, their processivity, and their rate of dissociation from microtubule ends. Traffic jams occurred when the motor density exceeded a critical value (density-induced jams) or when motor dissociation from the microtubule ends was so slow that it resulted in a pileup (bottleneck-induced jams). Through comparison of our experimental results with theoretical models and stochastic simulations, we characterized in detail under which conditions density- and bottleneck-induced traffic jams form or do not form. Our results indicate that transport kinesins, such as kinesin-1, may be evolutionarily adapted to avoid the formation of traffic jams by moving only with moderate processivity and dissociating rapidly from microtubule ends.
dc.language.isoen
dc.title.enMolecular crowding creates traffic jams of kinesin motors on microtubules
dc.typeCommunication dans un congrès avec actes
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.countryFR
bordeaux.title.proceedingDPG Fruhjahrstatung
bordeaux.conference.cityBerlin
bordeaux.peerReviewedoui
hal.identifierhal-00761068
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00761068v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=LEDUC,%20Cecile&PADBERG-GEHLE,%20Kathrin&VARGA,%20Vladimir&HELBING,%20Dirk&DIEZ,%20Stefan&rft.genre=proceeding


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