GIMÉNEZ, Pol; ANGUELA, Sergi; JUST-BORRAS, Arnau; PONS-MERCADÉ, Pere; VIGNAULT, Adeline; CANALS, Joan Miquel; TEISSEDRE, Pierre-Louis; ZAMORA, Fernando

doi:10.1016/j.lwt.2021.112871

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Article de revue

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LWT. 2022-01-15, vol. 154

Resumen en inglés

The aim of this paper is to develop a synthetic model reproducing more realistically the conditions of grape juice to study browning caused by laccase from Botrytis cinerea. The laccase browning kinetics were measured by monitoring the increase in absorbance at 420 nm over time in the presence of different substrates – one monophenol: 4-hydroxybenzoic acid; three orthodiphenols: caftaric acid, (+)-catechin and (−)-epicatechin; and one triphenol: gallic acid. The results indicate that orthodiphenols are better substrates than triphenols and that monophenols do not appear to be reactive. Of the orthodiphenols, (+)-catechin showed the greatest browning intensity, followed in decreasing order by (−)-epicatechin and caftaric acid. These results confirm that sulfur dioxide, ascorbic acid and glutathione really do protect grape juice against laccase browning. The effectiveness of ascorbic acid and glutathione also confirm that both antioxidants can be useful tools for reducing doses of sulfur dioxide in winemaking, especially when grey rot is present.< Leer menos

Palabras clave en inglés

Browning

Botrytis cinerea

Laccase

Synthetic model

URI

https://oskar-bordeaux.fr/handle/20.500.12278/180902

DOI

http://dx.doi.org/10.1016/j.lwt.2021.112871

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Oenologie - UMR 1366

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Development of a synthetic model to study browning caused by laccase activity from Botrytis cinerea

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Resumen en inglés

Palabras clave en inglés

URI

DOI

Centros de investigación