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Flavescence dorée phytoplasma enters insect cells by a clathrin-mediated endocytosis allowing infection of its insect vector

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorARRICAU-BOUVERY, Nathalie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDURET, Sybille
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDUBRANA, Marie-Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANUTO, Francesca
hal.structure.identifierBordeaux Imaging Center [BIC]
dc.contributor.authorBROCARD, Lysiane
hal.structure.identifierPlateforme Protéome [Bordeaux]
dc.contributor.authorCLAVEROL, Stéphane
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMALEMBIC-MAHER, Sylvie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFOISSAC, Xavier
dc.date.accessioned2023-05-09T10:21:34Z
dc.date.available2023-05-09T10:21:34Z
dc.date.issued2023-02-07
dc.identifier.issn2045-2322
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/173676
dc.description.abstractEnTo perform its propagative and circulative cycle into its insect vector, the flavescence dorée phytoplasma invades different cell types. Clathrin-mediated endocytosis is used by a wide range of bacteria to infect eukaryote cells. Among the insect proteins interacting with the phytoplasma adhesin VmpA, we identified the adaptor protein complex AP-1 and AP-2 suggesting that phytoplasmas could enter the insect cells via clathrin-mediated endocytosis. By infection assays of insect cells in culture, we showed that phytoplasmas entry into Drosophila S2 cells was more efficient than infection of the Euva cell line developed from the insect vector Euscelidius variegatus. Chlorpromazine, cytochalasin D and knockdown of clathrin heavy chain (chc) gene expression using RNA interference inhibited entry of phytoplasmas into S2 cells. During invasion of S2 cells, phytoplasmas were observed very closed to recombinant GFP-labelled clathrin light chain. To verify the role of clathrin in the insect colonization by phytoplasmas, RNAi was performed via artificial feeding of chc dsRNA by the vector E. variegatus. This decreased the expression of chc gene in the midgut and heads of E. variegatus. The chc lower expression correlated to a decreased of midgut and salivary gland cells colonization after the insects had ingested phytoplasmas from infected plants. In conclusion, results indicate that clathrin is important for the FD phytoplasma to enter insect cells and colonize its insect vector.
dc.language.isoen
dc.publisherNature Publishing Group
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectphytoplasme
dc.subjectMollicute phytopathogène
dc.subjectPathologie végétale
dc.subjectEndocytose dépendante de la clathrine
dc.subjectInfection
dc.subjectEpidemiologie
dc.subject.enFlavescence dorée phytoplasma
dc.subject.eninsect vector
dc.title.enFlavescence dorée phytoplasma enters insect cells by a clathrin-mediated endocytosis allowing infection of its insect vector
dc.typeArticle de revue
dc.identifier.doi10.1038/s41598-023-29341-1
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
bordeaux.journalScientific Reports
bordeaux.page2211
bordeaux.volume13
bordeaux.hal.laboratoriesBiologie du Fruit & Pathologie (BFP) - UMR 1332*
bordeaux.issue1
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-04062721
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04062721v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2023-02-07&rft.volume=13&rft.issue=1&rft.spage=2211&rft.epage=2211&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=ARRICAU-BOUVERY,%20Nathalie&DURET,%20Sybille&DUBRANA,%20Marie-Pierre&CANUTO,%20Francesca&BROCARD,%20Lysiane&rft.genre=article


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