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In vitro and in vivo characterization of a novel tricalcium silicate-based ink for bone regeneration using laser-assisted bioprinting

dc.rights.licenseopenen_US
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorTOUYA, Nicolas
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDEVUN, Mathilde
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorHANDSCHIN, Charles
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorCASENAVE, Sophia
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorAHMED OMAR, Naima
dc.contributor.authorGAUBERT, Alexandra
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDUSSERRE, Nathalie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorOLIVEIRA, Hugo
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorKEROUREDAN, Olivia
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDEVILLARD, Raphael
dc.date.accessioned2023-04-26T07:39:52Z
dc.date.available2023-04-26T07:39:52Z
dc.date.issued2022-03
dc.identifier.issn1758-5090en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/173196
dc.description.abstractEnGrafts aside, current strategies employed to overcome bone loss still fail to reproduce native tissue physiology. Among the emerging bioprinting strategies, laser-assisted bioprinting (LAB) offers very high resolution, allowing designing micrometric patterns in a contactless manner, providing a reproducible tool to test ink formulation. To this date, no LAB associated ink succeeded to provide a reproducible ad integrum bone regeneration on a murine calvaria critical size defect model. Using the Conformité Européenne (CE) approved BioRoot RCS® as a mineral addition to a collagen-enriched ink compatible with LAB, the present study describes the process of the development of a solidifying tricalcium silicate-based ink as a new bone repair promoting substrates in a LAB model. This ink formulation was mechanically characterized by rheology to adjust it for LAB. Printed aside stromal cells from apical papilla (SCAPs), this ink demonstrated a great cytocompatibility, with significant in vitro positive impact upon cell motility, and an early osteogenic differentiation response in the absence of another stimulus. Results indicated that the in vivo application of this new ink formulation to regenerate critical size bone defect tends to promote the formation of bone volume fraction without affecting the vascularization of the neo-formed tissue. The use of LAB techniques with this ink failed to demonstrate a complete bone repair, whether SCAPs were printed or not of at its direct proximity. The relevance of the properties of this specific ink formulation would therefore rely on the quantity applied in situ as a defect filler rather than its cell modulation properties observed in vitro. For the first time, a tricalcium silicate-based printed ink, based on rheological analysis, was characterized in vitro and in vivo, giving valuable information to reach complete bone regeneration through formulation updates. This LAB-based process could be generalized to normalize the characterization of candidate ink for bone regeneration.
dc.language.isoENen_US
dc.subject.entricalcium silicate
dc.subject.enink
dc.subject.enbioink
dc.subject.enlaser-assisted bioprinting
dc.subject.enbone repair
dc.subject.entissue engineering
dc.subject.enin vivo
dc.title.enIn vitro and in vivo characterization of a novel tricalcium silicate-based ink for bone regeneration using laser-assisted bioprinting
dc.title.alternativeBiofabricationen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1088/1758-5090/ac584ben_US
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologieen_US
bordeaux.journalBiofabricationen_US
bordeaux.volume14en_US
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026en_US
bordeaux.issue2en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.institutionINSERMen_US
bordeaux.institutionCHU de Bordeauxen_US
bordeaux.institutionInstitut Bergoniéen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-04081944
hal.version1
hal.date.transferred2023-04-26T07:40:00Z
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biofabrication&rft.date=2022-03&rft.volume=14&rft.issue=2&rft.eissn=1758-5090&rft.issn=1758-5090&rft.au=TOUYA,%20Nicolas&DEVUN,%20Mathilde&HANDSCHIN,%20Charles&CASENAVE,%20Sophia&AHMED%20OMAR,%20Naima&rft.genre=article


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