In vitro long term differentiation and functionality of three-dimensional bioprinted primary human hepatocytes: application for in vivo engraftment
| dc.rights.license | open | en_US |
| dc.contributor.author | CUVELLIER, Marie | |
| dc.contributor.author | ROSE, Sophie | |
| dc.contributor.author | EZAN, Frédéric | |
| dc.contributor.author | JARRY, Ulrich | |
| hal.structure.identifier | Bioingénierie tissulaire [BIOTIS] | |
| dc.contributor.author | OLIVEIRA, Hugo | |
| dc.contributor.author | BRUYÈRE, Arnaud | |
| dc.contributor.author | ROCHELLE, Christophe Drieu La | |
| dc.contributor.author | LEGAGNEUX, Vincent | |
| dc.contributor.author | LANGOUËT, Sophie | |
| dc.contributor.author | BAFFET, Georges | |
| dc.date.accessioned | 2023-04-04T12:51:36Z | |
| dc.date.available | 2023-04-04T12:51:36Z | |
| dc.date.issued | 2022-06 | |
| dc.identifier.issn | 1758-5090 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/172726 | |
| dc.description.abstractEn | In recent decades, 3D in vitro cultures of primary human hepatocytes (PHHs) have been increasingly developed to establish models capable of faithfully mimicking main liver functions. The use of 3D bioprinting, capable of recreating structures composed of cells embedded in matrix with controlled microarchitectures, is an emergent key feature for tissue engineering. In this work, we used an extrusion-based system to print PHH in a methacrylated gelatin (GelMa) matrix. PHH bioprinted in GelMa rapidly organized into polarized hollow spheroids and were viable for at least 28 d of culture. These PHH were highly differentiated with maintenance of liver differentiation genes over time, as demonstrated by transcriptomic analysis and functional approaches. The cells were polarized with localization of apico/canalicular regions, and displayed activities of phase I and II biotransformation enzymes that could be regulated by inducers. Furthermore, the implantation of the bioprinted structures in mice demonstrated their capability to vascularize, and their ability to maintain human hepatic specific functions for at least 28 d was illustrated by albumin secretion and debrisoquine metabolism. This model could hold great promise for human liver tissue generation and its use in future biotechnological developments. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | 3D culture | |
| dc.subject.en | 3D bioprinting | |
| dc.subject.en | primary human hepatocytes | |
| dc.subject.en | tissue engineering | |
| dc.subject.en | methacrylated gelatin | |
| dc.title.en | In vitro long term differentiation and functionality of three-dimensional bioprinted primary human hepatocytes: application for in vivo engraftment | |
| dc.title.alternative | Biofabrication | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1088/1758-5090/ac7825 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Médecine humaine et pathologie | en_US |
| bordeaux.journal | Biofabrication | en_US |
| bordeaux.volume | 14 | en_US |
| bordeaux.hal.laboratories | Bioingénierie Tissulaire (BioTis) - U1026 | en_US |
| bordeaux.issue | 3 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.institution | INSERM | en_US |
| bordeaux.institution | CHU de Bordeaux | en_US |
| bordeaux.institution | Institut Bergonié | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.export | false | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biofabrication&rft.date=2022-06&rft.volume=14&rft.issue=3&rft.eissn=1758-5090&rft.issn=1758-5090&rft.au=CUVELLIER,%20Marie&ROSE,%20Sophie&EZAN,%20Fr%C3%A9d%C3%A9ric&JARRY,%20Ulrich&OLIVEIRA,%20Hugo&rft.genre=article |
