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Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells

dc.rights.licenseopenen_US
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorKONG, Junhua
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorGARCIA, Virginie
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorZEHRAOUI, Enric
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorSTAMMITTI, Linda
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorGODINAUD, Ghislaine
IDREF: 225640449
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorRENAUD, Christel
dc.contributor.authorMAURY, Stéphane
dc.contributor.authorDELAUNAY, Alain
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorCLUZET, Stéphanie
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorLECOURIEUX, Fatma
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorLECOURIEUX, David
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorTEYSSIER, Emeline
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorGALLUSCI, Philippe
dc.date.accessioned2023-02-20T16:50:22Z
dc.date.available2023-02-20T16:50:22Z
dc.date.issued2022-07-15
dc.identifier.issn2073-4425en_US
dc.identifier.otherhttps://mdpi-res.com/d_attachment/genes/genes-13-01256/article_deploy/genes-13-01256-s001.zip?version=1657884457en_US
dc.identifier.otherhttps://www.ebi.ac.uk/ena/browser/view/PRJEB54585en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/172010
dc.description.abstractEnThrough its role in the regulation of gene expression, DNA methylation can participate in the control of specialized metabolite production. We have investigated the link between DNA methylation and anthocyanin accumulation in grapevine using the hypomethylating drug, zebularine and Gamay Teinturier cell suspensions. In this model, zebularine increased anthocyanin accumulation in the light, and induced its production in the dark. To unravel the underlying mechanisms, cell transcriptome, metabolic content, and DNA methylation were analyzed. The up-regulation of stress-related genes, as well as a decrease in cell viability, revealed that zebularine affected cell integrity. Concomitantly, the global DNA methylation level was only slightly decreased in the light and not modified in the dark. However, locus-specific analyses demonstrated a decrease in DNA methylation at a few selected loci, including a CACTA DNA transposon and a small region upstream from the UFGT gene, coding for the UDP glucose:flavonoid-3-O-glucosyltransferase, known to be critical for anthocyanin biosynthesis. Moreover, this decrease was correlated with an increase in UFGT expression and in anthocyanin content. In conclusion, our data suggest that UFGT expression could be regulated through DNA methylation in Gamay Teinturier, although the functional link between changes in DNA methylation and UFGT transcription still needs to be demonstrated.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enDNA methylation
dc.subject.enZebularine
dc.subject.enAnthocyanin
dc.title.enZebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/genes13071256en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalGenesen_US
bordeaux.page1256en_US
bordeaux.volume13en_US
bordeaux.hal.laboratoriesEcophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287en_US
bordeaux.issue7en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionINRAEen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.exportfalse
dc.rights.ccCC BYen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Genes&rft.date=2022-07-15&rft.volume=13&rft.issue=7&rft.spage=1256&rft.epage=1256&rft.eissn=2073-4425&rft.issn=2073-4425&rft.au=KONG,%20Junhua&GARCIA,%20Virginie&ZEHRAOUI,%20Enric&STAMMITTI,%20Linda&GODINAUD,%20Ghislaine&rft.genre=article


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