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dc.rights.licenseopenen_US
hal.structure.identifierUniversity of Victoria [Canada] [UVIC]
dc.contributor.authorPICARD, Katherine
dc.contributor.authorCORSI, Giorgio
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorDECOEUR, Fanny
hal.structure.identifierUniversity of Victoria [Canada] [UVIC]
dc.contributor.authorDI CASTRO, Maria Amalia
hal.structure.identifierUniversity of Victoria [Canada] [UVIC]
dc.contributor.authorBORDELEAU, Maude
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorPERSILLET, Marine
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorLAYE, Sophie
ORCID: 0000-0002-3843-1012
IDREF: 11366883X
hal.structure.identifierIRCCS Neuromed, Pozzilli, Italy
dc.contributor.authorLIMATOLA, Cristina
hal.structure.identifierUniversity of Victoria [Canada] [UVIC]
dc.contributor.authorTREMBLAY, Marie-Eve
hal.structure.identifierNeurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
dc.contributor.authorNADJAR, Agnes
dc.date.accessioned2022-12-09T10:47:25Z
dc.date.available2022-12-09T10:47:25Z
dc.date.created2022-09
dc.date.issued2022
dc.identifier.otherhttps://doi. org/10.1016/j.bbi.2022.09.016en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/170538
dc.description.abstractEnSleep is a natural physiological state, tightly regulated through several neuroanatomical and neurochemical systems, which is essential to maintain physical and mental health. Recent studies revealed that the functions of microglia, the resident immune cells of the brain, differ along the sleep-wake cycle. Inflammatory cytokines, such as interleukin-1β and tumor necrosis factor-α, mainly produced by microglia in the brain, are also well-known to promote sleep. However, the contributing role of microglia on sleep regulation remains largely elusive, even more so in females. Given the higher prevalence of various sleep disorders in women, we aimed to determine the role of microglia in regulating the sleep-wake cycle specifically in female mice. Microglia were depleted in adult female mice with inhibitors of the colony-stimulating factor 1 receptor (CSF1R) (PLX3397 or PLX5622), which is required for microglial population maintenance. This led to a 65-73% reduction of the microglial population, as confirmed by immunofluorescence staining against IBA1 (marker of microglia/macrophages) and TMEM119 (microglia-specific marker) in the reticular nucleus of the thalamus and primary motor cortex. The spontaneous sleep-wake cycle was evaluated at steady-state, during microglial homeostasis disruption and after complete microglial repopulation, upon cessation of treatment with the inhibitors of CSF1R, using electroencephalography (EEG) and electromyography (EMG). We found that microglia-depleted female mice spent more time in non-rapid eye movement (NREM) sleep and had an increased number of NREM sleep episodes, which was partially restored after microglial total repopulation. To determine whether microglia could regulate sleep locally by modulating synaptic transmission, we used patch clamp to record spontaneous activity of pyramidal neurons in the primary motor cortex, which showed an increase of excitatory synaptic transmission during the dark phase. These changes in neuronal activity were modulated by microglial depletion in a phase-dependent manner. Altogether, our results indicate that microglia are involved in the sleep regulation of female mice, further strengthening their potential implication in the development and/or progression of sleep disorders. Furthermore, our findings indicate that microglial repopulation can contribute to normalizing sleep alterations caused by their partial depletion.
dc.description.sponsorshipBordeaux Region Aquitaine Initiative for Neuroscience - ANR-10-LABX-0043en_US
dc.description.sponsorshipUniversity of Bordeaux Neurocampus Graduate School - ANR-17-EURE-0028en_US
dc.language.isoENen_US
dc.subject.enSleep
dc.subject.enEEG
dc.subject.enMicroglia depletion
dc.subject.enCSF1R
dc.subject.enNREM
dc.subject.enSynaptic plasticity
dc.title.enMicroglial homeostasis disruption modulates non-rapid eye movement sleep duration and neuronal activity in adult female mice.
dc.title.alternativeBrain Behav Immunen_US
dc.typeDocument de travail - Pré-publicationen_US
dc.identifier.doi10.1016/j.bbi.2022.09.016en_US
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]en_US
dc.identifier.pubmed36202169en_US
bordeaux.page153-164en_US
bordeaux.hal.laboratoriesNeurocentre Magendie - U1215en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.institutionINRAEen_US
bordeaux.teamPhysiopathologie de l'équilibre énergétique et obésitéen_US
bordeaux.identifier.funderIDFonds de recherche du Québecen_US
bordeaux.identifier.funderIDFondation pour la Recherche Médicaleen_US
bordeaux.identifier.funderIDConseil Régional Aquitaineen_US
bordeaux.identifier.funderIDMinistero della Saluteen_US
bordeaux.import.sourcepubmed
hal.identifierhal-03891569
hal.version1
hal.date.transferred2022-12-09T10:47:28Z
hal.exporttrue
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
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