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dc.rights.licenseopenen_US
hal.structure.identifierUniversité de Montréal [UdeM]
dc.contributor.authorKHLAIFIA, Abdessattar
hal.structure.identifierUniversité de Montréal [UdeM]
dc.contributor.authorHONORE, Eve
hal.structure.identifierUniversité de Montréal [UdeM]
hal.structure.identifierNeurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
dc.contributor.authorARTINIAN, Julien
hal.structure.identifierUniversité de Montréal [UdeM]
dc.contributor.authorLAPLANTE, Isabel
hal.structure.identifierUniversité de Montréal [UdeM]
dc.contributor.authorLACAILLE, Jean-Claude
dc.date.accessioned2022-11-10T10:31:42Z
dc.date.available2022-11-10T10:31:42Z
dc.date.issued2022-06-17
dc.identifier.issn1756-6606en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/170246
dc.description.abstractEnHippocampal CA1 parvalbumin-expressing interneurons (PV INs) play a central role in controlling principal cell activity and orchestrating network oscillations. PV INs receive excitatory inputs from CA3 Schaffer collaterals and local CA1 pyramidal cells, and they provide perisomatic inhibition. Schaffer collateral excitatory synapses onto PV INs express Hebbian and anti-Hebbian types of long-term potentiation (LTP), as well as elicit LTP of intrinsic excitability (LTPIE). LTPIE requires the activation of type 5 metabotropic glutamate receptors (mGluR5) and is mediated by downregulation of potassium channels Kv1.1. It is sensitive to rapamycin and thus may involve activation of the mammalian target of rapamycin complex 1 (mTORC1). LTPIE facilitates PV INs recruitment in CA1 and maintains an excitatory-inhibitory balance. Impaired CA1 PV INs activity or LTP affects network oscillations and memory. However, whether LTPIE in PV INs plays a role in hippocampus-dependent memory remains unknown. Here, we used conditional deletion of the obligatory component of mTORC1, the Regulatory-Associated Protein of mTOR (Raptor), to directly manipulate mTORC1 in PV INs. We found that homozygous, but not heterozygous, conditional knock-out of Rptor resulted in a decrease in CA1 PV INs of mTORC1 signaling via its downstream effector S6 phosphorylation assessed by immunofluorescence. In whole-cell recordings from hippocampal slices, repetitive firing of CA1 PV INs was impaired in mice with either homozygous or heterozygous conditional knock-out of Rptor. High frequency stimulation of Schaffer collateral inputs that induce LTPIE in PV INs of control mice failed to do so in mice with either heterozygous or homozygous conditional knock-out of Rptor in PV INs. At the behavioral level, mice with homozygous or heterozygous conditional knock-out of Rptor showed similar long-term contextual fear memory or contextual fear memory discrimination relative to control mice. Thus, mTORC1 activity in CA1 PV INs regulates repetitive firing and LTPIE but not consolidation of long-term contextual fear memory and context discrimination. Our results indicate that mTORC1 plays cell-specific roles in synaptic plasticity of hippocampal inhibitory interneurons that are differentially involved in hippocampus-dependent learning and memory. © 2022, The Author(s).
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.en4 Aminobutyric Acid Receptor
dc.subject.enKetamine
dc.subject.enMammalian Target Of Rapamycin Complex 1
dc.subject.enParvalbumin
dc.subject.enRegulatory Associated Protein Of Mtor
dc.subject.enS6 Kinase
dc.subject.enXylazine
dc.subject.enMammalian Target Of Rapamycin Complex 1
dc.subject.enParvalbumin
dc.subject.enAnimal Cell
dc.subject.enAnimal Experiment
dc.subject.enAnimal Tissue
dc.subject.enAntidromic Stimulation
dc.subject.enArticle
dc.subject.enControlled Study
dc.subject.enFear
dc.subject.enFear Conditioning Test
dc.subject.enFemale
dc.subject.enFiring Rate
dc.subject.enHippocampal Ca1 Region
dc.subject.enHippocampal Slice
dc.subject.enHippocampus Potential
dc.subject.enHomozygosity
dc.subject.enImmunofluorescence
dc.subject.enInterneuron
dc.subject.enLong Term Memory
dc.subject.enLong Term Potentiation
dc.subject.enMale
dc.subject.enMouse
dc.subject.enMtor Signaling
dc.subject.enNerve Excitability
dc.subject.enNonhuman
dc.subject.enProtein Function
dc.subject.enProtein Phosphorylation
dc.subject.enSchaffer Collateral Pathway
dc.subject.enWhole Cell Patch Clamp
dc.subject.enAnimal
dc.subject.enHippocampus
dc.subject.enInterneuron
dc.subject.enMammal
dc.subject.enMetabolism
dc.subject.enPhysiology
dc.subject.enSynapse
dc.subject.enAnimals
dc.subject.enFear
dc.subject.enHippocampus
dc.subject.enInterneurons
dc.subject.enLong-Term Potentiation
dc.subject.enMammals
dc.subject.enMechanistic Target Of Rapamycin Complex 1
dc.subject.enMice
dc.subject.enParvalbumins
dc.subject.enSynapses
dc.title.enmTORC1 function in hippocampal parvalbumin interneurons: regulation of firing and long‑term potentiation of intrinsic excitability but not long‑term contextual fear memory and context discrimination
dc.title.alternativeMol Brainen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1186/s13041-022-00941-8en_US
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]en_US
dc.identifier.pubmed35715811en_US
bordeaux.journalMolecular Brainen_US
bordeaux.page56en_US
bordeaux.volume15en_US
bordeaux.hal.laboratoriesNeurocentre Magendie - U1215en_US
bordeaux.issue1en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.teamCircuits neuronaux des apprentissages associatifsen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.identifier.funderIDCanadian Institutes of Health Researchen_US
bordeaux.identifier.funderIDUniversité de Montréalen_US
hal.identifierhal-03967115
hal.version1
hal.date.transferred2023-02-01T09:33:03Z
hal.exporttrue
dc.rights.ccCC BYen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20Brain&rft.date=2022-06-17&rft.volume=15&rft.issue=1&rft.spage=56&rft.epage=56&rft.eissn=1756-6606&rft.issn=1756-6606&rft.au=KHLAIFIA,%20Abdessattar&HONORE,%20Eve&ARTINIAN,%20Julien&LAPLANTE,%20Isabel&LACAILLE,%20Jean-Claude&rft.genre=article


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