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Model-Assisted Analysis of Sugar Metabolism throughout Tomato Fruit Development Reveals Enzyme and Carrier Properties in Relation to Vacuole Expansion.

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEAUVOIT, Bertrand
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCOLOMBIÉ, Sophie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMONIER, Antoine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorANDRIEU, Marie-Hélène
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBIAIS, Benoit
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBÉNARD, Camille
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCHÉNICLET, Catherine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDIEUAIDE-NOUBHANI, Martine
hal.structure.identifierInstitut de Mathématiques de Bordeaux [IMB]
dc.contributor.authorNAZARET, Christine
hal.structure.identifierPhysiopathologie mitochondriale
dc.contributor.authorMAZAT, Jean-Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
dc.date.issued2014
dc.identifier.issn1040-4651
dc.description.abstractEn: A kinetic model combining enzyme activity measurements and subcellular compartmentation was parameterized to fit the sucrose, hexose, and glucose-6-P contents of pericarp throughout tomato (Solanum lycopersicum) fruit development. The model was further validated using independent data obtained from domesticated and wild tomato species and on transgenic lines. A hierarchical clustering analysis of the calculated fluxes and enzyme capacities together revealed stage-dependent features. Cell division was characterized by a high sucrolytic activity of the vacuole, whereas sucrose cleavage during expansion was sustained by both sucrose synthase and neutral invertase, associated with minimal futile cycling. Most importantly, a tight correlation between flux rate and enzyme capacity was found for fructokinase and PPi-dependent phosphofructokinase during cell division and for sucrose synthase, UDP-glucopyrophosphorylase, and phosphoglucomutase during expansion, thus suggesting an adaptation of enzyme abundance to metabolic needs. In contrast, for most enzymes, flux rates varied irrespectively of enzyme capacities, and most enzymes functioned at <5% of their maximal catalytic capacity. One of the major findings with the model was the high accumulation of soluble sugars within the vacuole together with organic acids, thus enabling the osmotic-driven vacuole expansion that was found during cell division.
dc.language.isoen
dc.publisherAmerican Society of Plant Biologists (ASPB)
dc.title.enModel-Assisted Analysis of Sugar Metabolism throughout Tomato Fruit Development Reveals Enzyme and Carrier Properties in Relation to Vacuole Expansion.
dc.typeArticle de revue
dc.identifier.doi10.1105/tpc.114.127761
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalThe Plant cell
bordeaux.page3224-3242
bordeaux.volume26
bordeaux.issue8
bordeaux.peerReviewedoui
hal.identifierhal-01058814
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01058814v1
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