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Regulation of the fruit-specific PEP Carboxylase SlPPC2 promoter at early stages of tomato fruit development

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGUILLET, Carine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierFaculty of Agriculture
hal.structure.identifierDepartment of Clinical Laboratory Sciences
dc.contributor.authorABOUL-SOUD, Mourad
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorLE MENN, Aline
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorVIRON, Nicolas
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPRIBAT, Anne
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGERMAIN, Veronique
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJUST, Daniel
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALDET, Pierre
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorROUSSELLE, Patrick
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLEMAIRE, Martine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
dc.date.issued2012
dc.identifier.issn1932-6203
dc.description.abstractEnThe SlPPC2 phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) gene from tomato (Solanum lycopersicum) is differentially and specifically expressed in expanding tissues of developing tomato fruit. We recently showed that a 1966 bp DNA fragment located upstream of the ATG codon of the SlPPC2 gene (GenBank AJ313434) confers appropriate fruit-specificity in transgenic tomato. In this study, we further investigated the regulation of the SlPPC2 promoter gene by analysing the SlPPC2 cis-regulating region fused to either the firefly luciferase (LUC) or the beta-glucuronidase (GUS) reporter gene, using stable genetic transformation and biolistic transient expression assays in the fruit. Biolistic analyses of 5' SlPPC2 promoter deletions fused to LUC in fruits at the 8th day after anthesis revealed that positive regulatory regions are mostly located in the distal region of the promoter. In addition, a 5' UTR leader intron present in the 1966 bp fragment contributes to the proper temporal regulation of LUC activity during fruit development. Interestingly, the SlPPC2 promoter responds to hormones (ethylene) and metabolites (sugars) regulating fruit growth and metabolism. When tested by transient expression assays, the chimeric promoter: LUC fusion constructs allowed gene expression in both fruit and leaf, suggesting that integration into the chromatin is required for fruit-specificity. These results clearly demonstrate that SlPPC2 gene is under tight transcriptional regulation in the developing fruit and that its promoter can be employed to drive transgene expression specifically during the cell expansion stage of tomato fruit. Taken together, the SlPPC2 promoter offers great potential as a candidate for driving transgene expression specifically in developing tomato fruit from various tomato cultivars.
dc.language.isoen
dc.publisherPublic Library of Science
dc.title.enRegulation of the fruit-specific PEP Carboxylase SlPPC2 promoter at early stages of tomato fruit development
dc.typeArticle de revue
dc.identifier.doi10.1371/journal.pone.0036795
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Botanique
bordeaux.journalPLoS ONE
bordeaux.page1-11
bordeaux.volume7
bordeaux.issue5
bordeaux.peerReviewedoui
hal.identifierhal-01136957
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01136957v1
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