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Haplotype diversity of VvTFL1A gene and association with cluster traits in grapevine (V. vinifera)

hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierInstituto de Ciencias de la Vid y el Vino [ICVV]
dc.contributor.authorFERNANDEZ, Lucie
hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
dc.contributor.authorLE CUNFF, Loic
hal.structure.identifierInstituto de Ciencias de la Vid y el Vino [ICVV]
dc.contributor.authorTELLO, Javier
hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
dc.contributor.authorLACOMBE, Thierry
hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
dc.contributor.authorBOURSIQUOT, Jean-Michel
hal.structure.identifierBio21 Institute, Department of Genetics
dc.contributor.authorFOURNIER-LEVEL, Alexandre
hal.structure.identifierDpto. de Genética Molecular de Plantas
dc.contributor.authorBRAVO, Gema
hal.structure.identifierDomaine expérimental de Vassal [MONTP DOM VASSAL]
dc.contributor.authorLALET, Sandrine
hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
dc.contributor.authorTORREGROSA, Laurent
hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
dc.contributor.authorTHIS, Patrice
hal.structure.identifierInstituto de Ciencias de la Vid y el Vino [ICVV]
dc.contributor.authorMARTINEZ-ZAPATER, Jose-Miguel
dc.date.issued2014
dc.identifier.issn1471-2229
dc.description.abstractEnBackground [br/]Interaction between TERMINAL FLOWER 1 (TFL1) and LEAFY (LFY) seem to determine the inflorescence architecture in Arabidopsis. In a parallel way, overexpression of VvTFL1A, a grapevine TFL1 homolog, causes delayed flowering and production of a ramose cluster in the reiterated reproductive meristem (RRM) somatic variant of cultivar Carignan. To analyze the possible contribution of this gene to cluster phenotypic variation in a diversity panel of cultivated grapevine (Vitis vinifera L. subsp. vinifera) its nucleotide diversity was characterized and association analyses among detected sequence polymorphisms and phenology and cluster traits was carried out. [br/]Results [br/]A total of 3.6 kb of the VvTFL1A gene, including its promoter, was sequenced in a core collection of 140 individuals designed to maximize phenotypic variation at agronomical relevant traits. Nucleotide variation for VvTFL1A within this collection was higher in the promoter and intron sequences than in the exon regions; where few polymorphisms were located in agreement with a high conservation of coding sequence. Characterization of the VvTFL1A haplotype network identified three major haplogroups, consistent with the geographic origins and the use of the cultivars that could correspond to three major ancestral alleles or evolutionary branches, based on the existence of mutations in linkage disequilibrium. Genetic association studies with cluster traits revealed the presence of major INDEL polymorphisms, explaining 16%, 13% and 25% of flowering time, cluster width and berry weight, respectively, and also structuring the three haplogroups. [br/]Conclusions [br/]At least three major VvTFL1A haplogroups are present in cultivated grapevines, which are defined by the presence of three main polymorphism LD blocks and associated to characteristic phenotypic values for flowering time, cluster width and berry size. Phenotypic differences between haplogroups are consistent with differences observed between Eastern and Western grapevine cultivars and could result from the use of different genetic pools in the domestication process as well as different selection pressures on the development of table and wine cultivars, respectively. Altogether, these results are coherent with previous classifications of grapevine phenotypic diversity mainly based on cluster and berry morphotypes as well as with recent results on the structure of genetic diversity in cultivated grapevine.
dc.language.isoen
dc.publisherBioMed Central
dc.subject.enGrapevine
dc.subject.enGrape domestication
dc.subject.enBerry size
dc.subject.enPlant reproductive development
dc.subject.enInflorescence structure
dc.subject.enFlowering time
dc.title.enHaplotype diversity of VvTFL1A gene and association with cluster traits in grapevine (V. vinifera)
dc.typeArticle de revue
dc.identifier.doi10.1186/s12870-014-0209-3
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie du développement
bordeaux.journalBMC Plant Biology
bordeaux.page209
bordeaux.volume14
bordeaux.peerReviewedoui
hal.identifierhal-01123243
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01123243v1
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