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Resolving the Role of Plant Glutamate Dehydrogenase: II. Physiological Characterization of Plants Overexpressing the Two Enzyme Subunits Individually or Simultaneously.

hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorTERCÉ-LAFORGUE, Therese
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorBEDU, Magali
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorDARGEL-GRAFFIN, Céline
hal.structure.identifierFaculté des sciences
dc.contributor.authorDUBOIS, Frédéric
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
hal.structure.identifierUniversità degli studi di Parma = University of Parma [UNIPR]
dc.contributor.authorRESTIVO, Francesco M
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
dc.contributor.authorHIREL, Bertrand
dc.date.issued2013
dc.identifier.issn0032-0781
dc.description.abstractEnGlutamate dehydrogenase (GDH; EC 1.4.1.2) is able to carry out the deamination of glutamate in higher plants. In order to obtain a better understanding of the physiological function of GDH in leaves, transgenic tobacco (Nicotiana tabacum L.) plants were constructed that overexpress two genes from Nicotiana plumbaginifolia (GDHA and GDHB under the control of the Cauliflower mosiac virus 35S promoter), which encode the α- and β-subunits of GDH individually or simultaneously. In the transgenic plants, the GDH protein accumulated in the mitochondria of mesophyll cells and in the mitochondria of the phloem companion cells (CCs), where the native enzyme is normally expressed. Such a shift in the cellular location of the GDH enzyme induced major changes in carbon and nitrogen metabolite accumulation and a reduction in growth. These changes were mainly characterized by a decrease in the amount of sucrose, starch and glutamine in the leaves, which was accompanied by an increase in the amount of nitrate and Chl. In addition, there was an increase in the content of asparagine and a decrease in proline. Such changes may explain the lower plant biomass determined in the GDH-overexpressing lines. Overexpressing the two genes GDHA and GDHB individually or simultaneously induced a differential accumulation of glutamate and glutamine and a modification of the glutamate to glutamine ratio. The impact of the metabolic changes occurring in the different types of GDH-overexpressing plants is discussed in relation to the possible physiological function of each subunit when present in the form of homohexamers or heterohexamers.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subject.enCarbon metabolism
dc.subject.enGlutamate
dc.subject.enGlutamate dehydrogenase
dc.subject.enGlutamine
dc.subject.enSubunits
dc.subject.enTobacco
dc.title.enResolving the Role of Plant Glutamate Dehydrogenase: II. Physiological Characterization of Plants Overexpressing the Two Enzyme Subunits Individually or Simultaneously.
dc.typeArticle de revue
dc.identifier.doi10.1093/pcp/pct108
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalPlant and Cell Physiology
bordeaux.pagenp
bordeaux.volume54
bordeaux.issue10
bordeaux.peerReviewedoui
hal.identifierhal-01190621
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01190621v1
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