XU, Jiaxin; PASCUAL-BANULS, Laura; DESPLAT, Nelly; FAUROBERT, Mireille; GIBON, Yves; MOING, Annick; MAUCOURT, Mickaël; BALLIAS, P.; DEBORDE, Catherine; BOUCHET, Jean-Paul; BRUNEL, Dominique; LE PASLIER, Marie-Christine; CAUSSE, Mathilde

doi:10.17660/ActaHortic.2015.1099.100

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XU, Jiaxin
Génétique et Amélioration des Fruits et Légumes [GAFL]
College of horticulture

PASCUAL-BANULS, Laura
Génétique et Amélioration des Fruits et Légumes [GAFL]

DESPLAT, Nelly
Génétique et Amélioration des Fruits et Légumes [GAFL]

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Ce document a été publié dans

Acta Horticulturae. 2015, vol. 1099, p. 793-800

International Society for Horticultural Science

Résumé en anglais

Improvement of fruit quality traits is a major goal for tomato breeding. Deciphering the genetic diversity and inheritance of fruit quality components is thus necessary. For this purpose, we carried out a large multi-level omic experiment. Eight contrasted lines and 4 of their F-1 hybrids were phenotyped for fruit development traits. Fruit pericarp samples were analysed at 2 stages (cell expansion and orange ripe) and different scales: (1) untargeted profiling of major polar metabolites, (2) activities of 28 enzymes involved in primary metabolism, (3) proteome profiles revealed by 2D-PAGE and identification of 470 protein spots showing quantitative variations and (4) gene expression analysis by Digital Gene Expression. In parallel, the 8 lines were resequenced and more than 3 million SNPs identified when aligned on the reference tomato genome. This experiment allowed us to assess and compare the range of variability and inheritance mode of the metabolic traits and expression data. Correlation networks were constructed within and between levels of analysis to identify regulatory networks. Diversity of candidate genes could thus be analysed, relating the polymorphisms at the sequence levels with their expression.< Réduire

Mots clés

qualité du fruit

caractère polygénique

diversité génétique

développement du fruit

expression des gènes

activité enzymatique

tomate

solanum lycopersicum

protéome

transcriptome

métabolome

cellule de péricarpe

approche multi-omique

biologie des systèmes

profil métabolique

séquençage de gènes

profil protéomique

polymorphisme snp

Mots clés en anglais

fruit quality

genomic

SNP

genêtic variation

enzymic activity

tomato

systems biology