Bax mitochondrial relocation is linked to its phosphorylation and its interaction with Bcl-xL
GARENNE, David
Biologie du fruit et pathologie [BFP]
Centre National de la Recherche Scientifique [CNRS]
Biologie du fruit et pathologie [BFP]
Centre National de la Recherche Scientifique [CNRS]
GARENNE, David
Biologie du fruit et pathologie [BFP]
Centre National de la Recherche Scientifique [CNRS]
< Leer menos
Biologie du fruit et pathologie [BFP]
Centre National de la Recherche Scientifique [CNRS]
Idioma
en
Article de revue
Este ítem está publicado en
Microbial Cell. 2016, vol. 3, n° 12, p. 597-605
Shared Science Publishers OG
Resumen en inglés
The heterologous expression of Bax, and other Bcl-2 family members, in the yeast Saccharomyces cerevisiae, has proved to be a valuable reporter system to investigate the molecular mechanisms underlying their interaction ...Leer más >
The heterologous expression of Bax, and other Bcl-2 family members, in the yeast Saccharomyces cerevisiae, has proved to be a valuable reporter system to investigate the molecular mechanisms underlying their interaction with mitochondria. By combining the co-expression of Bax and Bcl-xL mutants with analyzes of their localization and interaction in mitochondria and post-mitochondrial supernatants, we showed that the ability of Bax and Bcl-xL to interact is dependent both on Bax phosphorylation - mimicked by a substitution S184D - and by Bax and Bcl-xL localization. This, and previous data, provide the molecular basis for a model of dynamic equilibrium for Bax localization and activation, regulated both by phosphorylation and Bcl-xL.< Leer menos
Palabras clave en inglés
Bax
Bcl-xL
mitochondria
phosphorylation
cytochrome c release
apoptosis
yeast (S.cerevisiae)
Orígen
Importado de HalCentros de investigación