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Viability and genetic stability of potato spindle tuber viroid mutants with indels in specific loops of the rod-like secondary structure.

hal.structure.identifierInstitute of Biochemistry and Biophysics
dc.contributor.authorWIĘSYK, Aneta
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierInstitute of Biochemistry and Biophysics
dc.contributor.authorZAGÓRSKI-OSTOJA, Włodzimierz
hal.structure.identifierInstitute of Biochemistry and Biophysics
dc.contributor.authorGÓRA-SOCHACKA, Anna
dc.date.issued2017
dc.identifier.issn0168-1702
dc.description.abstractEnMaintenance of the rod-like structure of potato spindle tuber viroid (PSTVd), which contains over 20 loops and bulges between double-stranded helices, is important for viroid biology. To study tolerance to modifications of the stem-loop structures and PSTVd capacity for mutation repair, we have created 6 mutants carrying 3-4 nucleotides deletions or insertions at three unique restriction sites, EagI, StyI and AvaII. Differences in the infectivity of these in vitro generated PSTVd mutants can result from where the mutations map, as well as from the extent to which the secondary structure of the molecule is affected. Deletion or insertion of 4 nucleotides at the EagI and StyI sites led to loss of infectivity. However, mutants with deletion (PSTVd-Ava-del) or insertion (PSTVd-Ava-in) of 3 nucleotides (221GAC223), at the AvaII site (loop 20) were viable but not genetically stable. In all analyzed plants, reversion to the wild type PSTVd-S23 sequence was observed for the PSTVd-Ava-in mutant a few weeks after agroinfiltration. Analysis of PSTVd-Ava-del progeny allowed the identification of 10 new sequence variants carrying various modifications, some of them having retained the original three nucleotide deletion at the AvaII site. Interestingly, other variants gained three nucleotides in the deletion site but did not revert to the original wild type sequence. The genetic stability of the progeny PSTVd-Ava-del sequence variants was evaluated in tomato leaves (early infection) and in both leaves and roots (late infection), respectively.
dc.language.isoen
dc.publisherElsevier
dc.subjectviroide phytopathogène
dc.subjectpotato spindle tuber viroid
dc.subjectmaladie des plantes
dc.subjectpathologie végétale
dc.subjectsanté des plantes
dc.subject.enagroinfiltration
dc.subject.engenetic stability
dc.subject.enindel mutants
dc.subject.eninfectivity
dc.subject.enviroid
dc.subject.enplant health
dc.title.enViability and genetic stability of potato spindle tuber viroid mutants with indels in specific loops of the rod-like secondary structure.
dc.typeArticle de revue
dc.identifier.doi10.1016/j.virusres.2017.07.024
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
bordeaux.journalVirus Research
bordeaux.page94-100
bordeaux.volume240
bordeaux.peerReviewedoui
hal.identifierhal-01602680
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01602680v1
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