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Increased expression of the NHL26 gene encoding a phloem plasmodesmal protein alters phloem export and sugar partitioning

hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
hal.structure.identifierUniversité Paris-Saclay
dc.contributor.authorVILAINE, Françoise
dc.contributor.authorKERCHEV, Pavel Ivanov
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
hal.structure.identifierUniversité Paris-Saclay
dc.contributor.authorCLÉMENT, Gilles
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBATAILLER, Brigitte
dc.contributor.authorCAYLA, Thibaud
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
hal.structure.identifierUniversité Paris-Saclay
dc.contributor.authorBILL, Laurence
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
hal.structure.identifierUniversité Paris-Saclay
dc.contributor.authorWOLFF, Nelly
hal.structure.identifierInstitut Jean-Pierre Bourgin [IJPB]
hal.structure.identifierUniversité Paris-Saclay
dc.contributor.authorDINANT, Sylvie
dc.date.issued2013
dc.date.conference2013
dc.description.abstractEnPhloem sugar transport is a complex process involving symplasmic and apoplasmic events. We characterized Arabidopsis overexpressor lines accumulating high levels of carbohydrates in mature leaves due to the ectopic expression of a phloem-specific gene encoding NHL26, a putative membrane protein. NHL26 overexpressor plants grew more slowly than wild-type plants and had a higher shoot biomass, contrasting with slower root growth and a lower seed yield. Interestingly, vein density was also modified in the source leaves, indicating downstream effects on vein development. Similar effects were observed when NHL26 was overexpressed in companion cells, under the control of a companion cell-specific promoter. The soluble sugar content of the phloem sap and sink organs was lower than that in the wild-type, providing evidence of a sugar export defect. This was confirmed in a phloem-export assay with CFDA. Leaf sugar accumulation was accompanied by higher organic acid, amino-acid and protein contents, whereas analysis of the metabolite profile by GC/MS of phloem sap exudate revealed no change in amino-acid or organic acid content, indicating a specific effect on the symplasmic export of sugars. NHL26 was found to be located in the phloem plasmodesmata and the endoplasmic reticulum. These findings reveal that NHL26 accumulation affects either the permeability of plasmodesmata or sugar signaling in companion cells, with a specific effect on sugar export.
dc.language.isoen
dc.subject.enphloem
dc.subject.enarabidopsis
dc.subject.encarbon allocation
dc.subject.enplasmodesmata
dc.title.enIncreased expression of the NHL26 gene encoding a phloem plasmodesmal protein alters phloem export and sugar partitioning
dc.typeCommunication dans un congrès
dc.subject.halSciences du Vivant [q-bio]
bordeaux.pagenp
bordeaux.conference.titlePlant Vascular Biology
bordeaux.countryFI
bordeaux.conference.cityHelsinki
bordeaux.peerReviewedoui
hal.identifierhal-01603025
hal.version1
hal.invitednon
hal.proceedingsoui
hal.conference.end2013
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01603025v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2013&rft.spage=np&rft.epage=np&rft.au=VILAINE,%20Fran%C3%A7oise&KERCHEV,%20Pavel%20Ivanov&CL%C3%89MENT,%20Gilles&BATAILLER,%20Brigitte&CAYLA,%20Thibaud&rft.genre=unknown


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