BENARD, Camille; GIBON, Yves

doi:10.1002/cppb.20003

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Licencia de uso del documento

BENARD, Camille
Biologie du fruit et pathologie [BFP]

GIBON, Yves
Biologie du fruit et pathologie [BFP]

Idioma

Chapitre d'ouvrage

Este ítem está publicado en

Current Protocols in Plant Biology, Current Protocols in Plant Biology. 2016p. np

John Wiley and Sons Inc

Resumen en inglés

The measurement of enzyme activities represents an important step towards the understanding of biological networks. Continuous or discontinuous assays can be used, as well as highly sensitive assays, depending on the abundance of the enzymes under study. To exemplify such methods, two protocols for phosphoenolpyruvate carboxylase activity (EC 4.1.1.31) in plant extracts are given. For this, an extraction protocol is also described. Then, an optimization protocol for enzyme assays using enzymatic, chemical, or biological standards is proposed. This protocol evaluates in one run the optimal extract dilution, the recovery of a standard, and the technical error in a given matrix. The interest of using biological standard in routine measurements is highlighted.< Leer menos

Palabras clave

métabolisme

physiologie végétale

Palabras clave en inglés

cycling assay

discontinuous assay

enzyme activity

optimization

PEPC

recovery

continuous assay

DOI

http://dx.doi.org/10.1002/cppb.20003

Proyecto ANR

Centre français de phénomique végétale - ANR-11-INBS-0012

Orígen

Importado de Hal

Centros de investigación

Biologie du Fruit & Pathologie (BFP) - UMR 1332