Characterization of a NADH-dependent glutamate dehydrogenase mutant of [i]Arabidopsis[/i] demonstrates the key role of this enzyme in root carbon and nitrogen metabolism
hal.structure.identifier | Biologie des Plantes et Innovation - UR UPJV 3900 [BIOPI] | |
dc.contributor.author | FONTAINE, Jean-Xavier | |
hal.structure.identifier | Institut Jean-Pierre Bourgin [IJPB] | |
dc.contributor.author | TERCÉ-LAFORGUE, Therese | |
hal.structure.identifier | Institut Jean-Pierre Bourgin [IJPB] | |
dc.contributor.author | ARMENGAUD, Patrick | |
hal.structure.identifier | Institut Jean-Pierre Bourgin [IJPB] | |
dc.contributor.author | CLÉMENT, Gilles | |
hal.structure.identifier | Unité de recherche en génomique végétale [URGV] | |
dc.contributor.author | RENOU, Jean-Pierre | |
hal.structure.identifier | Unité de recherche en génomique végétale [URGV] | |
dc.contributor.author | PELLETIER, Sandra | |
hal.structure.identifier | Ecologie et Dynamique des Systèmes Anthropisés - UMR CNRS 7058 UPJV [EDYSAN] | |
dc.contributor.author | CATTEROU, Manuella | |
hal.structure.identifier | Institut Jean-Pierre Bourgin [IJPB] | |
dc.contributor.author | AZZOPARDI, Marianne | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | GIBON, Yves | |
hal.structure.identifier | Rothamsted Research | |
dc.contributor.author | LEA, Peter J. | |
hal.structure.identifier | Institut Jean-Pierre Bourgin [IJPB] | |
dc.contributor.author | HIREL, Bertrand | |
hal.structure.identifier | Ecologie et Dynamique des Systèmes Anthropisés - UMR CNRS 7058 UPJV [EDYSAN] | |
dc.contributor.author | DUBOIS, Frédéric | |
dc.date.issued | 2012-10 | |
dc.identifier.issn | 1040-4651 | |
dc.description.abstractEn | The role of NADH-dependent glutamate dehydrogenase (GDH) was investigated by studying the physiological impact of a complete lack of enzyme activity in an Arabidopsis thaliana plant deficient in three genes encoding the enzyme. This study was conducted following the discovery that a third GDH gene is expressed in the mitochondria of the root companion cells, where all three active GDH enzyme proteins were shown to be present. A gdh1-2-3 triple mutant was constructed and exhibited major differences from the wild type in gene transcription and metabolite concentrations, and these differences appeared to originate in the roots. By placing the gdh triple mutant under continuous darkness for several days and comparing it to the wild type, the evidence strongly suggested that the main physiological function of NADH-GDH is to provide 2-oxoglutarate for the tricarboxylic acid cycle. The differences in key metabolites of the tricarboxylic acid cycle in the triple mutant versus the wild type indicated that, through metabolic processes operating mainly in roots, there was a strong impact on amino acid accumulation, in particular alanine, gamma-aminobutyrate, and aspartate in both roots and leaves. These results are discussed in relation to the possible signaling and physiological functions of the enzyme at the interface of carbon and nitrogen metabolism. | |
dc.language.iso | en | |
dc.publisher | American Society of Plant Biologists (ASPB) | |
dc.title.en | Characterization of a NADH-dependent glutamate dehydrogenase mutant of [i]Arabidopsis[/i] demonstrates the key role of this enzyme in root carbon and nitrogen metabolism | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1105/tpc.112.103689 | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | |
bordeaux.journal | The Plant cell | |
bordeaux.page | 4044-4065 | |
bordeaux.volume | 24 | |
bordeaux.issue | 10 | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-01019522 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Non spécifiée | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-01019522v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=The%20Plant%20cell&rft.date=2012-10&rft.volume=24&rft.issue=10&rft.spage=4044-4065&rft.epage=4044-4065&rft.eissn=1040-4651&rft.issn=1040-4651&rft.au=FONTAINE,%20Jean-Xavier&TERC%C3%89-LAFORGUE,%20Therese&ARMENGAUD,%20Patrick&CL%C3%89MENT,%20Gilles&RENOU,%20Jean-Pierre&rft.genre=article |
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