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Trans-species synthetic gene design allows resistance pyramiding and broad-spectrum engineering of virus resistance in plants

hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorBASTET, Anna
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorLEDERER, Baptiste
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorGIOVINAZZO, Nathalie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorARNOUX, Xavier
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGERMAN-RETANA, Sylvie
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorREINBOLD, Catherine
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorBRAULT, Véronique
hal.structure.identifierInstitut de Biologie Moléculaire des Plantes [IBMP]
dc.contributor.authorGARCIA, Damien
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorDJENNANE, Samia
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorGERSCH, Sophie
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorLEMAIRE, Olivier
hal.structure.identifierInstitut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
hal.structure.identifierLuminy Génétique et Biophysique des Plantes [LGBP]
dc.contributor.authorROBAGLIA, Christophe
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorGALLOIS, Jean-Luc
dc.date2018
dc.date.issued2018
dc.identifier.issn1467-7644
dc.description.abstractEnTo infect plants, viruses rely heavily on their host's machinery. Plant genetic resistances based on host factor modifications can be found among existing natural variability and are widely used for some but not all crops. While biotechnology can supply for the lack of natural resistance alleles, new strategies need to be developed to increase resistance spectra and durability without impairing plant development. Here, we assess how the targeted allele modification of the Arabidopsis thaliana translation initiation factor eIF4E1 can lead to broad and efficient resistance to the major group of potyviruses. A synthetic Arabidopsis thaliana eIF4E1 allele was designed by introducing multiple amino acid changes associated with resistance to potyvirus in naturally occurring Pisum sativum alleles. This new allele encodes a functional protein while maintaining plant resistance to a potyvirus isolate that usually hijacks eIF4E1. Due to its biological functionality, this synthetic allele allows, at no developmental cost, the pyramiding of resistances to potyviruses that selectively use the two major translation initiation factors, eIF4E1 or its isoform eIFiso4E. Moreover, this combination extends the resistance spectrum to potyvirus isolates for which no efficient resistance has so far been found, including resistance‐breaking isolates and an unrelated virus belonging to the Luteoviridae family. This study is a proof‐of‐concept for the efficiency of gene engineering combined with knowledge of natural variation to generate trans‐species virus resistance at no developmental cost to the plant. This has implications for breeding of crops with broad‐spectrum and high durability resistance using recent genome editing techniques.
dc.description.sponsorshipFacteurs cellulaires recrutés par les potyvirus pour leur transport intercellulaire : de nouvelles sources de résistance des plantes?
dc.language.isoen
dc.publisherWiley
dc.subject.enArabidopsis thaliana
dc.subject.ensynthetic allele
dc.subject.entranslational research
dc.subject.eneIF4E
dc.subject.enpotyvirus
dc.subject.enresistance
dc.title.enTrans-species synthetic gene design allows resistance pyramiding and broad-spectrum engineering of virus resistance in plants
dc.typeArticle de revue
dc.identifier.doi10.1111/pbi.12896
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Amélioration des plantes
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire
bordeaux.journalPlant Biotechnology Journal
bordeaux.page1569-1581
bordeaux.volume16
bordeaux.issue9
bordeaux.peerReviewedoui
hal.identifierhal-01852177
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01852177v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20Biotechnology%20Journal&rft.date=2018&rft.volume=16&rft.issue=9&rft.spage=1569-1581&rft.epage=1569-1581&rft.eissn=1467-7644&rft.issn=1467-7644&rft.au=BASTET,%20Anna&LEDERER,%20Baptiste&GIOVINAZZO,%20Nathalie&ARNOUX,%20Xavier&GERMAN-RETANA,%20Sylvie&rft.genre=article


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