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Modeling Protein Destiny in Developing Fruit

hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBELOUAH, Isma
hal.structure.identifierInstitut de Mathématiques de Bordeaux [IMB]
dc.contributor.authorNAZARET, Christine
hal.structure.identifierInteractions Plantes Pathogènes [IPP]
dc.contributor.authorPÉTRIACQ, Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPRIGENT, Sylvain
hal.structure.identifierUNIROUEN - UFR Santé [UNIROUEN UFR Santé]
dc.contributor.authorBÉNARD, Camille
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorMENGIN, Virginie
hal.structure.identifierGénétique Quantitative et Evolution - Le Moulon (Génétique Végétale) [GQE-Le Moulon]
dc.contributor.authorBLEIN-NICOLAS, Melisande
dc.contributor.authorDENTON, Alisandra
hal.structure.identifierGénétique Quantitative et Evolution - Le Moulon (Génétique Végétale) [GQE-Le Moulon]
dc.contributor.authorBALLIAU, Thierry
dc.contributor.authorAUGÉ, Ségolène
hal.structure.identifierLaboratoire de Génétique Cellulaire [LGC]
dc.contributor.authorBOUCHEZ, Olivier
hal.structure.identifierPhysiopathologie mitochondriale
dc.contributor.authorMAZAT, Jean-Pierre
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorSTITT, Mark
hal.structure.identifierBioEcon Sci Ctr, Inst Bot & Mol Genet
dc.contributor.authorUSADEL, Björn
hal.structure.identifierGénétique Quantitative et Evolution - Le Moulon (Génétique Végétale) [GQE-Le Moulon]
dc.contributor.authorZIVY, Michel
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEAUVOIT, Bertrand
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCOLOMBIE, Sophie
dc.date.issued2019-06-28
dc.identifier.issn0032-0889
dc.description.abstractEnProtein synthesis and degradation are essential processes that regulate cell status. Because labeling in bulky organs, such as fruits, is difficult, we developed a modeling approach to study protein turnover at the global scale in developing tomato (Solanum lycopersicum) fruit. Quantitative data were collected for transcripts and proteins during fruit development. Clustering analysis showed smaller changes in protein abundance compared to mRNA abundance. Furthermore, protein and transcript abundance were poorly correlated, and the coefficient of correlation decreased during fruit development and ripening, with transcript levels decreasing more than protein levels. A mathematical model with one ordinary differential equation was used to estimate translation (kt) and degradation (kd) rate constants for almost 2,400 detected transcript-protein pairs and was satisfactorily fitted for over a thousand pairs. The model predicted median values of about 2 min for the translation of a protein, and a protein lifetime of approximately 11 days. The constants were validated and inspected for biological relevance. Proteins involved in protein synthesis had higher kt and kd values, indicating that the protein machinery is particularly flexible. Our model also predicts that protein concentration is more strongly affected by the rate of translation than that of degradation.
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
dc.language.isoen
dc.publisherOxford University Press ; American Society of Plant Biologists
dc.title.enModeling Protein Destiny in Developing Fruit
dc.typeArticle de revue
dc.identifier.doi10.1104/pp.19.00086
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Bio-Informatique, Biologie Systémique [q-bio.QM]
bordeaux.journalPlant Physiology
bordeaux.page1709-1724
bordeaux.volume180
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-02327302
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02327302v1
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