When a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence dorée epidemics in Europe
FILIPPIN, Luisa
Consiglio per la Ricerca in Agricoltura e l’analisi dell’economia agraria = Council for Agricultural Research and Economics [CREA]
Consiglio per la Ricerca in Agricoltura e l’analisi dell’economia agraria = Council for Agricultural Research and Economics [CREA]
ANGELINI, Elisa
Consiglio per la Ricerca in Agricoltura e l’analisi dell’economia agraria = Council for Agricultural Research and Economics [CREA]
< Reduce
Consiglio per la Ricerca in Agricoltura e l’analisi dell’economia agraria = Council for Agricultural Research and Economics [CREA]
Language
en
Article de revue
This item was published in
PLoS Pathogens. 2020, vol. 16, n° 3, p. e1007967
Public Library of Science
English Abstract
Flavescence doré e (FD) is a European quarantine grapevine disease transmitted by the Deltocephalinae leafhopper Scaphoideus titanus. Whereas this vector had been introduced from North America, the possible European origin ...Read more >
Flavescence doré e (FD) is a European quarantine grapevine disease transmitted by the Deltocephalinae leafhopper Scaphoideus titanus. Whereas this vector had been introduced from North America, the possible European origin of FD phytoplasma needed to be challenged and correlated with ecological and genetic drivers of FD emergence. For that purpose , a survey of genetic diversity of these phytoplasmas in grapevines, S. titanus, black alders, alder leafhoppers and clematis were conducted in five European countries. Out of 132 map genotypes, only 11 were associated to FD outbreaks, three were detected in cle-matis, whereas 127 were detected in alder trees, alder leafhoppers or in grapevines out of FD outbreaks. Most of the alder trees were found infected, including 8% with FD genotypes M6, M38 and M50, also present in alders neighboring FD-free vineyards and vineyard-free areas. The Macropsinae Oncopsis alni could transmit genotypes unable to achieve transmission by S. titanus, while the Deltocephalinae Allygus spp. and Orientus ishidae transmitted M38 and M50 that proved to be compatible with S. titanus. Variability of vmpA and vmpB adhesin-like genes clearly discriminated 3 genetic clusters. Cluster Vmp-I grouped genotypes only transmitted by O. alni, while clusters Vmp-II and-III grouped genotypes transmitted by Deltocephalinae leafhoppers. Interestingly, adhesin repeated domains evolved independently in cluster Vmp-I, whereas in clusters Vmp-II and-III showed recent duplications. Latex beads coated with various ratio of VmpA of clusters II and I, showed that cluster PLOS PATHOGENS PLOS Pathogens | https://doi.org/10.1371/journal.ppat.Read less <
Keywords
Flavescence dorée de la vigne
Fruit
santé des plantes
Bactérie phytopathogène
Vigne Vitis vinifera
Plante fruitière
Pathologie végétale
Mollicute phytopathogène
English Keywords
Phytoplasma
Adhesin
Epidemiology
Insect vector
Grapevine yellows
European Project
European South Eastern European SEE-ERANET Balkan Initiative network “Global epidemiology of phytoplasma diseases of economic importance in Southeast Europe” grant ID 06-100003110724
Origin
Hal importedCollections