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High prevalence of integrative and conjugative elements encoding transcription activator-like effector repeats in mycoplasma hominis

hal.structure.identifierCHU Bordeaux
hal.structure.identifierUSC IHMC - Infections humaines à mycoplasmes et à chlamydiae
hal.structure.identifierCentre national français de référence pour les campylobacters et les hélicobactéries [Bordeaux]
dc.contributor.authorMEYGRET, Alexandra
hal.structure.identifierCHU Bordeaux
hal.structure.identifierUSC IHMC - Infections humaines à mycoplasmes et à chlamydiae
hal.structure.identifierCentre national français de référence pour les campylobacters et les hélicobactéries [Bordeaux]
dc.contributor.authorPEUCHANT, Olivia
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorDORDET-FRISONI, Emilie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIRAND-PUGNET, Pascal
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorCITTI, Christine
hal.structure.identifierCHU Bordeaux
hal.structure.identifierUSC IHMC - Infections humaines à mycoplasmes et à chlamydiae
hal.structure.identifierCentre national français de référence pour les campylobacters et les hélicobactéries [Bordeaux]
dc.contributor.authorBEBEAR, Cécile
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEVEN, Laure
hal.structure.identifierCHU Bordeaux
hal.structure.identifierUSC IHMC - Infections humaines à mycoplasmes et à chlamydiae
hal.structure.identifierCentre national français de référence pour les campylobacters et les hélicobactéries [Bordeaux]
dc.contributor.authorPEREYRE, Sabine
dc.date.issued2019-10-18
dc.identifier.issn1664-302X
dc.description.abstractEnIntegrative and conjugative elements (ICEs) are modular mobile genetic elements that can disseminate through excision, circularization, and transfer. Mycoplasma ICEs have recently been found distributed among some mycoplasma species and there is accumulating evidence that they play a pivotal role in horizontal gene transfers. The occurrence of ICEs has not been documented in Mycoplasma hominis, a human urogenital pathogen responsible for urogenital infections, neonatal infections and extragenital infections. In this study, we searched for, characterized, and compared ICEs by genome analyses of 12 strains of M. hominis. ICEs of 27-30 kb were found in one or two copies in seven of the 12 M. hominis strains sequenced. Only five of these ICEs seemed to be functional, as assessed by detection of circular forms of extrachromosomal ICE. Moreover, the prevalence of ICEs in M. hominis was estimated to be 45% in a collection of 120 clinical isolates of M. hominis, including 27 tetracyclineresistant tet(M)-positive isolates. The proportion of ICEs was not higher in isolates carrying the tet(M) gene, suggesting that ICEs are not involved in tetracycline resistance. Notably, all M. hominis ICEs had a very similar structure, consisting of a 4.0-5.1 kb unusual module composed of five to six juxtaposed CDSs. All the genes forming this module were specific to M. hominis ICEs as they had no homologs in other mycoplasma ICEs. In each M. hominis ICE, one to three CDSs encode proteins that share common structural features with transcription activator-like (TAL) effectors involved in polynucleotide recognition and signal transduction in symbiotic plant pathogen bacteria. The conserved and specific structure of M. hominis ICEs and the high prevalence in clinical strains suggest that these ICEs may confer a selective advantage for the physiology or pathogenicity of this human pathogenic bacterium. These data open the way for further studies aiming at unraveling horizontal gene transfers and virulence factors in M. hominis.
dc.language.isoen
dc.publisherFrontiers Media
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enMycoplasma hominis
dc.subject.enhorizontal gene transfer
dc.subject.enintegrative and conjugative element
dc.subject.entet(M)
dc.subject.entranscription activator-like effector
dc.title.enHigh prevalence of integrative and conjugative elements encoding transcription activator-like effector repeats in mycoplasma hominis
dc.typeArticle de revue
dc.identifier.doi10.3389/fmicb.2019.02385
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences de l'environnement
bordeaux.journalFrontiers in Microbiology
bordeaux.page2385
bordeaux.volume10
bordeaux.peerReviewedoui
hal.identifierhal-02617744
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02617744v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers%20in%20Microbiology&rft.date=2019-10-18&rft.volume=10&rft.spage=2385&rft.epage=2385&rft.eissn=1664-302X&rft.issn=1664-302X&rft.au=MEYGRET,%20Alexandra&PEUCHANT,%20Olivia&DORDET-FRISONI,%20Emilie&SIRAND-PUGNET,%20Pascal&CITTI,%20Christine&rft.genre=article


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