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A chitinase is required for Xylella fastidiosa colonization of its insect and plant hosts

hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPolicy and Management
dc.contributor.authorLABROUSSAA, Fabien
hal.structure.identifierPlant and Microbial Biology
dc.contributor.authorIONESCU, Michael
hal.structure.identifierUniversity of California [Berkeley] [UC Berkeley]
hal.structure.identifierPolicy and Management
dc.contributor.authorZEILINGER, Adam R.
hal.structure.identifierPlant and Microbial Biology
dc.contributor.authorLINDOW, Steven E.
hal.structure.identifierUniversity of California [Berkeley] [UC Berkeley]
hal.structure.identifierPolicy and Management
dc.contributor.authorALMEIDA, Rodrigo P. P.
dc.date.issued2017
dc.identifier.issn1350-0872
dc.description.abstractEnXylella fastidiosa colonizes the xylem network of host plant species as well as the foregut of its required insect vectors to ensure efficient propagation. Disease management strategies remain inefficient due to a limited comprehension of the mechanisms governing both insect and plant colonization. It was previously shown that X. fastidiosa has a functional chitinase (ChiA), and that chitin likely serves as a carbon source for this bacterium. We expand on that research, showing that a chiA mutant strain is unable to grow on chitin as the sole carbon source. Quantitative PCR assays allowed us to detect bacterial cells in the foregut of vectors after pathogen acquisition; populations of the wild-type and complemented mutant strain were both significantly larger than the chiA mutant strain 10 days, but not 3 days, post acquisition. These results indicate that adhesion of the chiA mutant strain to vectors may not be impaired, but that cell multiplication is limited. The mutant was also affected in its transmission by vectors to plants. In addition, the chiA mutant strain was unable to colonize host plants, suggesting that the enzyme has other substrates associated with plant colonization. Lastly, ChiA requires other X. fastidiosa protein(s) for its in vitro chitinolytic activity. The observation that the chiA mutant strain is not able to colonize plants warrants future attention to be paid to the substrates for this enzyme.
dc.language.isoen
dc.publisherMicrobiology Society
dc.subjectXylella fastidiosa
dc.subjectsymbiosis
dc.subject.enplant pathogen
dc.subject.eninsect-microbe interactions
dc.subject.enchitinase
dc.title.enA chitinase is required for Xylella fastidiosa colonization of its insect and plant hosts
dc.typeArticle de revue
dc.identifier.doi10.1099/mic.0.000438
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences de l'environnement
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalMicrobiology
bordeaux.page502-509
bordeaux.volume163
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-02620205
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02620205v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Microbiology&rft.date=2017&rft.volume=163&rft.issue=4&rft.spage=502-509&rft.epage=502-509&rft.eissn=1350-0872&rft.issn=1350-0872&rft.au=LABROUSSAA,%20Fabien&IONESCU,%20Michael&ZEILINGER,%20Adam%20R.&LINDOW,%20Steven%20E.&ALMEIDA,%20Rodrigo%20P.%20P.&rft.genre=article


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