Important genetic diversity of Candidatus Phytoplasma solani’ related strains associated with bois noir grapevine yellows and planthoppers in Azerbaijan
Langue
en
Article de revue
Ce document a été publié dans
European Journal of Plant Pathology. 2018, vol. 151, n° 4, p. 937-946
Springer Verlag
Résumé en anglais
Bois noir (BN) is an important grapevine yellows endemic to the Euro-Mediterranean basin caused by ‘<em>Candidatus</em> Phytoplasma solani’ (‘Ca. P. solani’), a non culturable plant pathogenic Mollicute. Bois noir symptoms ...Lire la suite >
Bois noir (BN) is an important grapevine yellows endemic to the Euro-Mediterranean basin caused by ‘<em>Candidatus</em> Phytoplasma solani’ (‘Ca. P. solani’), a non culturable plant pathogenic Mollicute. Bois noir symptoms could be associated with ‘Ca. P. solani’ in two Azerbaijanian vineyards where disease incidence and severity were recorded for five local <em>Vitis vinifera</em> cultivars. In order to gain insight into the epidemiology of Bois noir in Azerbaijan, ‘Ca. P. solani’ isolates infecting plants were characterized by multi-locus sequence analysis and their secY and stamp gene sequences compared to that of the strains detected in other plants and in local Cixiidae planthoppers. Genotypes were determined for two non-ribosomal house-keeping genes, namely tuf and secY, as well as two variable markers namely Stamp and mleP1 genes, that respectively encode the antigenic membrane protein AMP and a 2-Hydroxycarboxylate transporter. The Azerbaijanian BN phytoplasma isolates corresponded to three tufB and secY genotypes. A finer differentiation of Azerbaijanian ‘Ca. P. solani’ isolates was obtained with mleP1 as five different mleP1 genetic variants were found. Finally, Stamp gene allowed differentiating four new genotypes in grapevine among the 10 new Stamp genotypes detected in various plants in Azerbaijan. The preliminary survey for infected insects conducted in northern Azerbaijan, led to the identification of <em>Hyalesthes obsoletus</em> and <em>Reptalus noah</em>i as potential vectors for two ‘Ca. P. solani’ new genotypes phylogenetically distant from the known genetic clusters. Altogether these results indicate an important genetic diversity of BN phytoplasmas in Azerbaijan that certainly result from spread through local insect vectors.< Réduire
Mots clés
Cixiidae
MLSA
Tuf
Mots clés en anglais
Vitis vinifera
secY
mleP1
stamp
plant pathogen
Origine
Importé de halUnités de recherche