NtTPN1: a RPP8‐like R gene required for Potato virus Y‐induced veinal necrosis in tobacco
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | MICHEL, Vincent | |
hal.structure.identifier | Imperial Tobacco Group | |
dc.contributor.author | JULIO, Emilie | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | CANDRESSE, Thierry | |
hal.structure.identifier | Imperial Tobacco Group | |
dc.contributor.author | COTUCHEAU, Julien | |
hal.structure.identifier | Imperial Tobacco Group | |
dc.contributor.author | DECORPS, Christophe | |
hal.structure.identifier | Imperial Tobacco Group | |
dc.contributor.author | VOLPATTI, Roxane | |
hal.structure.identifier | Unité de Pathologie Végétale [PV] | |
dc.contributor.author | MOURY, Benoît | |
hal.structure.identifier | Institut de Génétique, Environnement et Protection des Plantes [IGEPP] | |
hal.structure.identifier | Fédération Nationale des Producteurs de Plants de Pomme de Terre [FN3PT] | |
dc.contributor.author | GLAIS, Laurent | |
hal.structure.identifier | Imperial Tobacco Group | |
dc.contributor.author | DORLHAC DE BORNE, François | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | DECROOCQ, Véronique | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | GERMAN-RETANA, Sylvie | |
dc.date.issued | 2018-08 | |
dc.identifier.issn | 0960-7412 | |
dc.description.abstractEn | Potato virus Y is one of the most damaging viruses of tobacco. In particular, aggressive necrotic strains (PVYN) lead to considerable losses in yield. The main source of resistance against PVY is linked to the va locus. However, va‐overcoming PVY isolates inducing necrotic symptoms were observed in several countries. In this context, it is important to find va‐independent protection strategies. In a previous study, the phenotyping of 162 tobacco varieties revealed ten accessions that do not carry the va allele and do not exhibit typical PVYN‐induced veinal necrosis. Despite the absence of necrotic symptoms, normal viral accumulation in these plants suggests a va‐independent mechanism of tolerance to PVYN‐induced systemic veinal necrosis. Fine mapping of the genetic determinant(s) was performed in a segregating F2 population. The tolerance trait is inherited as a single recessive gene and allelism tests demonstrated that eight of the ten tolerant varieties carry the same determinant. Anchoring the linkage map to the tobacco genome physical map allowed the identification of a RPP8‐like R gene, called NtTPN1 (for <em>Nicotiana tabacum</em> Tolerance to PVY‐induced Necrosis1), with the same single‐nucleotide polymorphism in the eight tolerant accessions. Functional assays using homozygous NtTPN1 EMS mutants confirmed the role of NtTPN1 in the tolerance phenotype. PVYN‐induced systemic veinal necrosis in tobacco likely represents an inefficient defense response with HR‐like characteristics. The identification of NtTPN1 opens breeding options to minimize the impact of emerging and so far uncontrolled va‐breaking necrotic PVY isolates. | |
dc.description.sponsorship | Développement d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004 | |
dc.language.iso | en | |
dc.publisher | Wiley | |
dc.subject | tolerance | |
dc.subject | veinal necrosis | |
dc.subject.en | RPP8-like gene | |
dc.subject.en | PVY | |
dc.subject.en | Potyvirus | |
dc.subject.en | resistance | |
dc.subject.en | HR | |
dc.subject.en | R gene | |
dc.subject.en | Nicotiana tabacum | |
dc.title.en | NtTPN1: a RPP8‐like R gene required for Potato virus Y‐induced veinal necrosis in tobacco | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1111/tpj.13980 | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie | |
dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie | |
dc.subject.hal | Sciences du Vivant [q-bio]/Sciences agricoles | |
bordeaux.journal | The Plant Journal | |
bordeaux.page | 700-714 | |
bordeaux.volume | 95 | |
bordeaux.issue | 4 | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-02623766 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Internationale | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-02623766v1 | |
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