CANDRESSE, Thierry; THEIL, Sébastien; FAURE, Chantal; MARAIS, Armelle

doi:10.1007/s00705-017-3587-7

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CANDRESSE, Thierry
Biologie du fruit et pathologie [BFP]

THEIL, Sébastien
Biologie du fruit et pathologie [BFP]

FAURE, Chantal
Biologie du fruit et pathologie [BFP]

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Archives of Virology. 2018, vol. 163, n° 1, p. 277-280

Springer Verlag

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The present work reports the discovery and the complete genome sequencing of a novel member of the genus Vitivirus in the family Betaflexiviridae (subfamily Trivirinae) from a symptomless grapevine of unknown variety from Portugal. Total RNAs extracted from phloem scrapings were sequenced using Illumina technology. Bioinformatic analysis of the RNA-seq data revealed a mixed infection involving three viruses and two viroids in addition to a novel vitivirus. Completion and analysis of the genome sequence (7446 nt excluding the polyA tail) showed a typical vitivirus genomic organization. Phylogenetic analysis of the various ORFs clearly showed the new virus to belong in the genus Vitivirus, but sequence divergence firmly establishes it as a member of a new species, for which the name "Grapevine virus H" is proposed.< Leer menos

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Determination of the complete genomic sequence of grapevine virus H, a novel vitivirus infecting grapevine.

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