Characterization of GMO or glyphosate effects on the composition of maize grain and maize-based diet for rat feeding
COUMOUL, Xavier
Toxicité environnementale, cibles thérapeutiques, signalisation cellulaire [T3S - UMR_S 1124]
< Reduce
Toxicité environnementale, cibles thérapeutiques, signalisation cellulaire [T3S - UMR_S 1124]
Language
en
Article de revue
This item was published in
Metabolomics. 2018, vol. 14, n° 36, p. 1-12
Springer Verlag
English Abstract
<strong>Introduction</strong> In addition to classical targeted biochemical analyses, metabolomic analyses seem pertinent to reveal expected as well as unexpected compositional differences between plant genetically modified ...Read more >
<strong>Introduction</strong> In addition to classical targeted biochemical analyses, metabolomic analyses seem pertinent to reveal expected as well as unexpected compositional differences between plant genetically modified organisms (GMO) and non-GMO samples. Data previously published in the existing literature led to divergent conclusions on the effect of maize transgenes on grain compositional changes and feeding effects. Therefore, a new study examining field-grown harvested products and feeds derived from them remains useful. <strong>Objectives</strong> Our aim was to use a metabolomics approach to characterize grain and grain-based diet compositional changes for two GMO events, one involving Bacillus thuringiensis toxin to provide insect resistance and the other one conferring herbicide tolerance by detoxification of glyphosate. We also investigated the potential compositional modifications induced by the use of a glyphosate-based herbicide on the transgenic line conferring glyphosate tolerance. <strong>Results</strong> The majority of statistically significant differences in grain composition, evidenced by the use of 1H-NMR profiling of polar extracts and LC-ESI-QTOF-MS profiling of semi-polar extracts, could be attributed to the combined effect of genotype and environment. In comparison, transgene and glyphosate effects remained limited in grain for the compound families studied. Some but not all compositional changes observed in grain were also detected in grain-based diets formulated for rats. <strong>Conclusion</strong> Only part of the data previously published in the existing literature on maize grains of plants with the same GMO events could be reproduced in our experiment. All spectra have been deposited in a repository freely accessible to the public. Our grain and diet characterization opened the way for an in depth study of the effects of these diets on rat health.Read less <
Keywords
Metabolomics
Grain
English Keywords
Maize
GMO
Rat diet
ANR Project
Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation
Centre français de phénomique végétale - ANR-11-INBS-0012
Centre français de phénomique végétale - ANR-11-INBS-0012
Origin
Hal importedCollections