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A complex virome unveiled by deep sequencing analysis of RNAs from a French Pinot Noir grapevine exhibiting strong leafroll symptoms.

hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorBEUVE, Monique
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorHILY, Jean-Michel
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorALLIAUME, Antoine
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorREINBOLD, Catherine
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorLE MAGUET, Jean
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorHERRBACH, Etienne
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorLEMAIRE, Olivier
dc.date.issued2018
dc.identifier.issn0304-8608
dc.description.abstractEnWe have characterized the virome of a grapevine Pinot Noir accession (P70) that displayed, over the year, very stable and strong leafroll symptoms. For this, we have used two extraction methods (dsRNA and total RNA) coupled with the high throughput sequencing (HTS) Illumina technique. While a great disparity in viral sequences were observed, both approaches gave similar results, revealing a very complex infection status. Five virus and viroid isolates [Grapevine leafroll-associated viruse-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine rupestris stem pitting-associated virus (GRSPaV), Hop stunt viroid (HSVd) and Grapevine yellow speckle viroid 1 (GYSVd1)] were detected in P70 with a grand total of eleven variants being identified and de novo assembled. A comparison between both extraction methods regarding their power to detect viruses and the ease of genome assembly is also provided.
dc.language.isoen
dc.publisherSpringer Verlag
dc.subject.envirome
dc.subject.enGrapevine rupestris stem pitting-associated virus
dc.subject.enHop stunt viroid
dc.subject.enGrapevine yellow speckle viroid 1
dc.title.enA complex virome unveiled by deep sequencing analysis of RNAs from a French Pinot Noir grapevine exhibiting strong leafroll symptoms.
dc.typeArticle de revue
dc.identifier.doi10.1007/s00705-018-3949-9
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
bordeaux.journalArchives of Virology
bordeaux.page2937-2946
bordeaux.volume163
bordeaux.peerReviewedoui
hal.identifierhal-02628897
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02628897v1
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