A Plant-produced candidate subunit vaccine reduces shedding of Enterohemorrhagic <em>Escherichia col</em>i in ruminants
| hal.structure.identifier | Department of Biology | |
| hal.structure.identifier | Agriculture and Agri-Food [AAFC] | |
| dc.contributor.author | MILETIC, Sean | |
| hal.structure.identifier | Lethbridge Research Development Centre | |
| hal.structure.identifier | Georg-August-University = Georg-August-Universität Göttingen | |
| dc.contributor.author | HUENERBERG, Martin | |
| hal.structure.identifier | Agriculture and Agri-Food [AAFC] | |
| dc.contributor.author | KALDIS, Angelo | |
| hal.structure.identifier | Agriculture and Agri-Food [AAFC] | |
| dc.contributor.author | MACDONALD, Jacqueline | |
| hal.structure.identifier | Agriculture and Agri-Food [AAFC] | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | LEUTHREAU, Antoine | |
| hal.structure.identifier | Lethbridge Research and Development Centre | |
| dc.contributor.author | MCALLISTER, Tim | |
| hal.structure.identifier | University of Western Ontario [UWO] | |
| hal.structure.identifier | Agriculture and Agri-Food [AAFC] | |
| dc.contributor.author | MENASSA, Rima | |
| dc.date.issued | 2017 | |
| dc.identifier.issn | 1860-6768 | |
| dc.description.abstractEn | Enterohemorrhagic Escherichia coli (EHEC) are commonly present in the gastrointestinal tract of cattle and cause serious infectious disease in humans. Immunizing cattle against EHEC is a promising strategy to decrease the risk of food contamination; however, veterinary vaccines against EHEC such as Econiche have not been widely adopted by the agricultural industry, and have been discontinued, prompting the need for more cost-effective EHEC vaccines. The objective of this project is to develop a platform to produce plant-made antigens for oral vaccination of ruminants against EHEC. Five recombinant proteins were designed as vaccine candidates and expressed transiently in Nicotiana benthamiana and transplastomically in Nicotiana tabacum. Three of these EHEC proteins, NleA, Stx2b, and a fusion of EspA accumulated when transiently expressed. Transient protein accumulation was the highest when EHEC proteins were fused to an elastin-like polypeptide (ELP) tag. In the transplastomic lines, EspA accumulated up to 479mgkg(-1) in lyophilized leaf material. Sheep that were administered leaf tissue containing recombinant EspA shed less E. coli O157:H7 when challenged, as compared to control animals. These results suggest that plant-made, transgenic EspA has the potential to reduce EHEC shedding in ruminants. | |
| dc.language.iso | en | |
| dc.publisher | Wiley-VCH Verlag | |
| dc.subject | EHEC | |
| dc.subject.en | E. coli O157:H7 | |
| dc.subject.en | molecular farming | |
| dc.subject.en | oral vaccine | |
| dc.subject.en | veterinary vaccines | |
| dc.title.en | A Plant-produced candidate subunit vaccine reduces shedding of Enterohemorrhagic <em>Escherichia col</em>i in ruminants | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1002/biot.201700405 | |
| dc.subject.hal | Sciences du Vivant [q-bio] | |
| bordeaux.journal | Biotechnology Journal | |
| bordeaux.volume | 12 | |
| bordeaux.issue | 10 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-02628926 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-02628926v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biotechnology%20Journal&rft.date=2017&rft.volume=12&rft.issue=10&rft.eissn=1860-6768&rft.issn=1860-6768&rft.au=MILETIC,%20Sean&HUENERBERG,%20Martin&KALDIS,%20Angelo&MACDONALD,%20Jacqueline&LEUTHREAU,%20Antoine&rft.genre=article |
Fichier(s) constituant ce document
| Fichiers | Taille | Format | Vue |
|---|---|---|---|
|
Il n'y a pas de fichiers associés à ce document. |
|||