Afficher la notice abrégée

The plant WEE1 kinase is involved in checkpoint control activation in nematode induced galls.

hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
dc.contributor.authorCABRAL, Danila
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
hal.structure.identifierAin Shams University [ASU]
dc.contributor.authorBANORA, Mohamed Youssef
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
hal.structure.identifierLaboratório de Interação Molecular Planta-Praga
dc.contributor.authorDIJAIR ANTONINO, José
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
hal.structure.identifierEmbrapa Recursos Genéticos e Biotecnologia
dc.contributor.authorRODIUC, Natalia
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
dc.contributor.authorVIEIRA, Paulo
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
hal.structure.identifierEmbrapa Recursos Genéticos e Biotecnologia
dc.contributor.authorR. COELHO, Roberta
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCHEVALIER, Christian
hal.structure.identifierDepartment of Plant Biotechnology and Genetics
hal.structure.identifierFlanders Institute for Biotechnology
dc.contributor.authorEEKHOUT, Thomas
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
dc.contributor.authorENGLER, Gilbert
hal.structure.identifierDepartment of Plant Biotechnology and Genetics
hal.structure.identifierFlanders Institute for Biotechnology
dc.contributor.authorDE VEYLDER, Lieven
hal.structure.identifierEmbrapa Recursos Genéticos e Biotecnologia
dc.contributor.authorGROSSI-DE-SA, Maria Fatima
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
dc.contributor.authorDE ALMEIDA ENGLER, Janice
dc.date.issued2020
dc.identifier.issn0028-646X
dc.description.abstractEnGalls induced by plant-parasitic nematodes involve a hyperactivation of the plant mitotic and endocycle machinery for their profit. Dedifferentiation of host root cells includes drastic cellular and molecular readjustments. In such background, potential DNA damage in the genome of gall cells is eminent. We questioned if DNA damage checkpoints activation followed by DNA repair occurred, or was eventually circumvented, in nematode-induced galls. Galls display transcriptional activation of the DNA damage checkpoint kinase WEE1, correlated with its protein localization in the nuclei. The promoter of the stress marker gene SMR7 was evaluated under the WEE1-knockout background. Drugs inducing DNA damage and a marker for DNA repair, PARP1 were used to understand mechanisms that might cope with DNA damage in galls. Our functional study revealed that gall cells lacking WEE1 conceivably entered mitosis prematurely disturbing the cell cycle despite the loss of genome integrity. The disrupted nuclei phenotype in giant cells hinted to the accumulation of mitotic defects. As well, WEE1-knockout in Arabidopsis and downregulation in tomato repressed infection and reproduction of root-knot nematodes. Together with data on DNA damaging drugs, we suggest a conserved function for WEE1 controlling a G1/S cell cycle arrest in response to replication defect in galls.
dc.language.isoen
dc.publisherWiley
dc.subjectcell cycle
dc.subjectgalls
dc.subject.enArabidopsis thaliana
dc.subject.enSolanum lycopersicum
dc.subject.enWEE1 kinase
dc.subject.encheckpoint control
dc.subject.enroot-knot nematode
dc.title.enThe plant WEE1 kinase is involved in checkpoint control activation in nematode induced galls.
dc.typeArticle de revue
dc.identifier.doi10.1111/nph.16185
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalNew Phytologist
bordeaux.page430-447
bordeaux.volume225
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-02629362
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02629362v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=New%20Phytologist&rft.date=2020&rft.volume=225&rft.issue=1&rft.spage=430-447&rft.epage=430-447&rft.eissn=0028-646X&rft.issn=0028-646X&rft.au=CABRAL,%20Danila&BANORA,%20Mohamed%20Youssef&DIJAIR%20ANTONINO,%20Jos%C3%A9&RODIUC,%20Natalia&VIEIRA,%20Paulo&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée