Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance.
| hal.structure.identifier | International Livestock Research Institute [CGIAR, Nairobi] [ILRI] | |
| dc.contributor.author | SCHIECK, Elise | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | LARTIGUE, Carole | |
| hal.structure.identifier | Universität Bern / University of Bern [UNIBE] | |
| dc.contributor.author | FREY, Joachim | |
| hal.structure.identifier | Department of Biological Sciences | |
| dc.contributor.author | VOZZA, Nicolas | |
| hal.structure.identifier | Institute of Functional and Applied Anatomy | |
| dc.contributor.author | HEGERMANN, Jan | |
| hal.structure.identifier | Department of Food Science | |
| dc.contributor.author | MILLER, Rachel A | |
| hal.structure.identifier | Washington University School of Medicine [Saint Louis, MO] | |
| dc.contributor.author | VALGUARNERA, Ezequiel | |
| hal.structure.identifier | International Livestock Research Institute [CGIAR, Nairobi] [ILRI] | |
| dc.contributor.author | MURIUKI, Cecilia | |
| hal.structure.identifier | University of Veterinary Medicine Hannover | |
| dc.contributor.author | MEENS, Jochen | |
| hal.structure.identifier | International Livestock Research Institute [CGIAR, Nairobi] [ILRI] | |
| dc.contributor.author | NENE, Vish | |
| hal.structure.identifier | International Livestock Research Institute [CGIAR, Nairobi] [ILRI] | |
| dc.contributor.author | NAESSENS, Jan | |
| hal.structure.identifier | Université de Lausanne = University of Lausanne [UNIL] | |
| dc.contributor.author | WEBER, Johann | |
| hal.structure.identifier | University of Alberta | |
| dc.contributor.author | LOWARY, Todd L | |
| hal.structure.identifier | J. Craig Venter Institute [La Jolla, USA] [JCVI] | |
| dc.contributor.author | VASHEE, Sanjay | |
| hal.structure.identifier | Department of Biological Sciences | |
| dc.contributor.author | FELDMAN, Mario F | |
| hal.structure.identifier | International Livestock Research Institute [CGIAR, Nairobi] [ILRI] | |
| dc.contributor.author | JORES, Joerg | |
| dc.date.issued | 2016 | |
| dc.identifier.issn | 0950-382X | |
| dc.description.abstractEn | Mycoplasma mycoides subsp. capri (Mmc) and subsp. mycoides (Mmm) are important ruminant pathogens worldwide causing diseases such as pleuropneumonia, mastitis and septicaemia. They express galactofuranose residues on their surface, but their role in pathogenesis has not yet been determined. The M. mycoides genomes contain up to several copies of the glf gene, which encodes an enzyme catalyzing the last step in the synthesis of galactofuranose. We generated a deletion of the glf gene in a strain of Mmc using genome transplantation and tandem repeat endonuclease coupled cleavage (TREC) with yeast as an intermediary host for the genome editing. As expected, the resulting YCp1.1-Δglf strain did not produce the galactofuranose containing glycans as shown by immunoblots and immuno-electronmicroscopy employing a galactofuranose specific monoclonal antibody. The mutant lacking galactofuranose exhibited a decreased growth rate and a significantly enhanced adhesion to small ruminant cells. The mutant was also "leaking" as revealed by a β-galactosidase-based assay employing a membrane impermeable substrate. These findings indicate that galactofuranose-containing polysaccharides conceal adhesins and are important for membrane integrity. Unexpectedly, the mutant strain showed increased serum resistance. | |
| dc.language.iso | en | |
| dc.publisher | Wiley | |
| dc.subject | galactan | |
| dc.subject | galactofuranose | |
| dc.subject | membrane | |
| dc.subject | synthetic genomics | |
| dc.subject.en | Mycoplasma mycoides | |
| dc.subject.en | UDP-galactopyranose mutase | |
| dc.subject.en | adhesion | |
| dc.subject.en | carbohydrate | |
| dc.title.en | Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance. | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1111/mmi.13213 | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie animale/Médecine vétérinaire et santé animal | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie animale | |
| bordeaux.journal | Molecular Microbiology | |
| bordeaux.page | 55-70 | |
| bordeaux.volume | 99 | |
| bordeaux.issue | 1 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-02631324 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-02631324v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20Microbiology&rft.date=2016&rft.volume=99&rft.issue=1&rft.spage=55-70&rft.epage=55-70&rft.eissn=0950-382X&rft.issn=0950-382X&rft.au=SCHIECK,%20Elise&LARTIGUE,%20Carole&FREY,%20Joachim&VOZZA,%20Nicolas&HEGERMANN,%20Jan&rft.genre=article |
Files in this item
| Files | Size | Format | View |
|---|---|---|---|
|
There are no files associated with this item. |
|||