Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines.
OLMOS, Antonio
Instituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research [IVIA]
< Réduire
Instituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research [IVIA]
Langue
en
Article de revue
Ce document a été publié dans
Archives of Virology. 2014, vol. 159, n° 8, p. 2103-2107
Springer Verlag
Résumé en anglais
Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % ...Lire la suite >
Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5' extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic.< Réduire
Mots clés en anglais
Trichovirus
Next-generation sequencing
Diversity
Detection
Origine
Importé de halUnités de recherche