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A novel grapevine badnavirus is associated with the Roditis leaf discoloration disease.

hal.structure.identifierFaculty of Agriculture, Forestry and Natural Environment, School of Agriculture
dc.contributor.authorMALIOGKA, Varvara I
hal.structure.identifierInstituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research [IVIA]
dc.contributor.authorOLMOS, Antonio
hal.structure.identifierFaculty of Agriculture, Forestry and Natural Environment, School of Agriculture
dc.contributor.authorPAPPI, Polyxeni G
hal.structure.identifierFaculty of Agriculture, Forestry and Natural Environment, School of Agriculture
dc.contributor.authorLOTOS, Leonidas
hal.structure.identifierFaculty of Agriculture, Forestry and Natural Environment, School of Agriculture
dc.contributor.authorEFTHIMIOU, Konstantinos
hal.structure.identifierFaculty of Agriculture & Food Technology
dc.contributor.authorGRAMMATIKAKI, Garyfalia
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierFaculty of Agriculture, Forestry and Natural Environment, School of Agriculture
dc.contributor.authorKATIS, Nikolaos I
hal.structure.identifierResearch Institute of Viticulture and Oenology
dc.contributor.authorAVGELIS, Apostolos D
dc.date.issued2015
dc.identifier.issn0168-1702
dc.description.abstractEnRoditis leaf discoloration (RLD), a graft-transmissible disease of grapevine, was first reported in Greece in the 1980s. Even though various native grapevine viruses were identified in the affected vines, the etiology of the disease remained unknown. In the present study, we used an NGS platform for sequencing siRNAs from a twenty-year old Roditis vine showing typical RLD symptoms. Analysis of the NGS data revealed the presence of various known grapevine viruses and viroids as well as a hitherto uncharacterized DNA virus. The circular genome of the new virus was fully reassembled. It is 6988 nts long and includes 4 open reading frames (ORFs). ORF1, ORF2 and ORF4 code for proteins with unknown functions while ORF3 encodes a polyprotein with motifs related to the replication, encapsidation and movement of the virus. Phylogenetic analysis classified the novel virus within the genus Badnavirus, with closest relationship to Fig badnavirus 1. Further studies showed that the new badnavirus is closely related with the RLD disease and the provisional name grapevine Roditis leaf discoloration-associated virus (GRLDaV) is proposed. Our findings extend the number of DNA viruses identified in grapevine, further drawing attention to the potential importance of this virus group on grapevine pathology.
dc.language.isoen
dc.publisherElsevier
dc.subject.enBadnavirus
dc.subject.enGrapevine
dc.subject.enNext generation sequencing
dc.subject.enRoditis leaf discoloration
dc.subject.enSmall interfering RNAs
dc.title.enA novel grapevine badnavirus is associated with the Roditis leaf discoloration disease.
dc.typeArticle de revue
dc.identifier.doi10.1016/j.virusres.2015.03.003
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie
bordeaux.journalVirus Research
bordeaux.page47-55
bordeaux.volume203
bordeaux.peerReviewedoui
hal.identifierhal-02636096
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02636096v1
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