Afficher la notice abrégée

Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP(®) using reverse transcription-recombinase polymerase amplification.

hal.structure.identifierAgdia Inc
dc.contributor.authorZHANG, Shulu
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorRAVELONANDRO, Michel
hal.structure.identifierAgdia Inc
dc.contributor.authorRUSSELL, Paul
hal.structure.identifierAgdia Inc
dc.contributor.authorMCOWEN, Nathan
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBRIARD, Pascal
hal.structure.identifierAgdia Inc
dc.contributor.authorBOHANNON, Seven
hal.structure.identifierAgdia Inc
dc.contributor.authorVRIENT, Albert
dc.date.issued2014
dc.identifier.issn0166-0934
dc.description.abstractEnPlum pox virus (PPV) causes the most destructive viral disease known as plum pox or Sharka disease in stone fruit trees. As an important regulated pathogen, detection of PPV is thus of critical importance to quarantine and eradication of the spreading disease. In this study, the innovative development of two AmplifyRP(®) tests is reported for a rapid isothermal detection of PPV using reverse transcription-recombinase polymerase amplification. In an AmplifyRP(®) test, all specific recombination and amplification reactions occur at a constant temperature without thermal cycling and the test results are either recorded in real-time with a portable fluorescence reader or displayed using a lateral flow strip contained inside an amplicon detection chamber. The major improvement of this assay is that the entire test from sample preparation to result can be completed in as little as 20min and can be performed easily both in laboratories and in the field. The results from this study demonstrated the ability of the AmplifyRP(®) technique to detect all nine PPV strains (An, C, CR, D, EA, M, Rec, T, or W). Among the economic benefits to pathogen surveys is the higher sensitivity of the AmplifyRP(®) to detect PPV when compared to the conventional ELISA and ImmunoStrip(®) assays. This is the first report describing the use of such an innovative technique to detect rapidly plant viruses affecting perennial crops.
dc.language.isoen
dc.publisherElsevier
dc.subjectDiagnosis
dc.subject.enAmplifyRP(®)
dc.subject.enIsothermal amplification
dc.subject.enPlum pox virus
dc.subject.enRecombinase polymerase amplification
dc.title.enRapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP(®) using reverse transcription-recombinase polymerase amplification.
dc.typeArticle de revue
dc.identifier.doi10.1016/j.jviromet.2014.06.026
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
bordeaux.journalJournal of Virological Methods
bordeaux.page114-120
bordeaux.volume207
bordeaux.peerReviewedoui
hal.identifierhal-02636742
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02636742v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Virological%20Methods&rft.date=2014&rft.volume=207&rft.spage=114-120&rft.epage=114-120&rft.eissn=0166-0934&rft.issn=0166-0934&rft.au=ZHANG,%20Shulu&RAVELONANDRO,%20Michel&RUSSELL,%20Paul&MCOWEN,%20Nathan&BRIARD,%20Pascal&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée