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Rapid identification of causal mutations in tomato EMS populations via mapping-by-sequencing.

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGARCIA, Virginie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBRES, Cécile
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJUST, Daniel
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFERNANDEZ, Lucie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorWONG JUN TAI, Virginie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUXION, Jean-Philippe
hal.structure.identifierEtude du Polymorphisme des Génomes Végétaux [EPGV]
dc.contributor.authorLE PASLIER, Marie-Christine
hal.structure.identifierEtude du Polymorphisme des Génomes Végétaux [EPGV]
dc.contributor.authorBERARD, Aurélie
hal.structure.identifierEtude du Polymorphisme des Génomes Végétaux [EPGV]
dc.contributor.authorBRUNEL, Dominique
hal.structure.identifierOsaka University
dc.contributor.authorAOKI, Koh
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorALSEEKH, Saleh
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorFERNIE, Alisdair R
hal.structure.identifierSchool of Biological Sciences
dc.contributor.authorFRASER, Paul D
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
dc.date.issued2016
dc.identifier.issn1750-2799
dc.description.abstractEnThe tomato is the model species of choice for fleshy fruit development and for the Solanaceae family. Ethyl methanesulfonate (EMS) mutants of tomato have already proven their utility for analysis of gene function in plants, leading to improved breeding stocks and superior tomato varieties. However, until recently, the identification of causal mutations that underlie particular phenotypes has been a very lengthy task that many laboratories could not afford because of spatial and technical limitations. Here, we describe a simple protocol for identifying causal mutations in tomato using a mapping-by-sequencing strategy. Plants displaying phenotypes of interest are first isolated by screening an EMS mutant collection generated in the miniature cultivar Micro-Tom. A recombinant F2 population is then produced by crossing the mutant with a wild-type (WT; non-mutagenized) genotype, and F2 segregants displaying the same phenotype are subsequently pooled. Finally, whole-genome sequencing and analysis of allele distributions in the pools allow for the identification of the causal mutation. The whole process, from the isolation of the tomato mutant to the identification of the causal mutation, takes 6-12 months. This strategy overcomes many previous limitations, is simple to use and can be applied in most laboratories with limited facilities for plant culture and genotyping.
dc.language.isoen
dc.publisherNature Publishing Group
dc.title.enRapid identification of causal mutations in tomato EMS populations via mapping-by-sequencing.
dc.typeArticle de revue
dc.identifier.doi10.1038/nprot.2016.143
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
bordeaux.journalNature Protocols
bordeaux.page2401-2418
bordeaux.volume11
bordeaux.issue12
bordeaux.peerReviewedoui
hal.identifierhal-02638680
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02638680v1
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