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Remarkable reproducibility of enzyme activity profiles in tomato fruits grown under contrasting environments provides a roadmap for studies of fruit metabolism.

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBIAIS, Benoit
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBÉNARD, Camille
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEAUVOIT, Bertrand
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCOLOMBIE, Sophie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPRODHOMME, Duyên
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMENARD, Guillaume
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBERNILLON, Stéphane
hal.structure.identifierUniversity of Oxford
dc.contributor.authorGEHL, Bernadette
hal.structure.identifierUnité de recherche Plantes et Systèmes de Culture Horticoles [PSH]
dc.contributor.authorGAUTIER, Hélène
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALLIAS, Patricia
hal.structure.identifierUniversité Sciences et Technologies - Bordeaux 1 [UB]
dc.contributor.authorMAZAT, Jean-Pierre
hal.structure.identifierUniversity of Oxford
dc.contributor.authorSWEETLOVE, Lee J
hal.structure.identifierUnité de recherche Plantes et Systèmes de Culture Horticoles [PSH]
dc.contributor.authorGÉNARD, Michel
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
dc.date.issued2014
dc.identifier.issn0032-0889
dc.description.abstractEnTo assess the influence of the environment on fruit metabolism, Tomato (Solanum lycopersicum) cv. Moneymaker plants were grown under contrasting conditions (optimal for commercial production, water limitation or shading) and locations. Samples were harvested at nine stages of development and 36 enzymes activities of central metabolism were measured, as well as protein and major metabolites such as hexoses, sucrose, starch, organic acids and amino acids. The most remarkable result was the high reproducibility of enzyme time-courses throughout development irrespective of conditions or location. Hierarchical clustering of enzyme activities also revealed tight relationships between metabolic pathways and phases of development. Thus, cell division was characterized by high hexokinases, pyruvate kinase and TCA-cycle enzymes, indicating ATP-production as a priority, whereas cell expansion was characterized by enzymes involved in the lower part of glycolysis, suggesting a metabolic reprogramming towards anapleurosis. As expected enzymes involved in the accumulation of sugars, citrate and glutamate were strongly increased at ripening. However, a group of enzymes involved in ATP-production, which is probably fueled by starch degradation, were also increased. Metabolites appeared more sensitive than enzymes to the environment, although such differences tended to decrease at ripening. The integration of enzyme and metabolite data obtained under contrasting growth conditions using principal component analysis suggests that at the exception of alanine amino-transferase and glutamate, as well as malate dehydrogenase and malate, there are no links between single enzyme activities and metabolite fluxes or levels.
dc.language.isoen
dc.publisherOxford University Press ; American Society of Plant Biologists
dc.title.enRemarkable reproducibility of enzyme activity profiles in tomato fruits grown under contrasting environments provides a roadmap for studies of fruit metabolism.
dc.typeArticle de revue
dc.identifier.doi10.1104/pp.113.231241
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPlant Physiology
bordeaux.page1204-1221
bordeaux.volume164
bordeaux.peerReviewedoui
hal.identifierhal-02639362
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02639362v1
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