A new eIF4E1 allele characterized by RNAseq data mining is associated with resistance to PVY in tomato albeit with a low durability
Langue
en
Article de revue
Ce document a été publié dans
Journal of General Virology. 2016, vol. 97, n° 11, p. 3063-3072
Microbiology Society
Résumé en anglais
Allele mining on susceptibility factors offers opportunities to find new sources of resistance among crop wild relatives for breeding purposes. As a proof of concept, we used available RNAseq data to investigate polymorphisms ...Lire la suite >
Allele mining on susceptibility factors offers opportunities to find new sources of resistance among crop wild relatives for breeding purposes. As a proof of concept, we used available RNAseq data to investigate polymorphisms among the four tomato genes encoding translation initiation factors (eIF4E1 and eIF4E2, eIFiso4E and the related gene New Cap-binding Protein -nCBP) to look for new potential resistance alleles to potyviruses. By analyzing polymorphism among RNAseq data obtained for 20 tomato accessions, ten belonging to the cultivated type <em>Solanum lycopersicum</em> and 10 belonging to the closest related wild species<em> Solanum pimpinellifolium</em>, we isolated one new eIF4E1 allele, in the <em>Solanum pimpinellifolium</em> LA0411 accession, which encodes a potential new resistance allele, mainly due to a polymorphism associated with an amino acid change within eIF4E1 region II. We confirmed that this new allele, pot1², is indeed associated with resistance to PVY, although with a restricted resistance spectrum and a very low durability potential. This suggests that mutations occurring in eIF4E region II only may not be sufficient to provide efficient and durable resistance in plants. However, our study emphasizes the opportunity brought by RNAseq data to mine for new resistance alleles. Moreover, this approach could be extended to seek for putative new resistance alleles by screening for variant forms of susceptibility genes encoding plant host proteins known to interact with viral proteins< Réduire
Mots clés
Potato virus Y
Mots clés en anglais
allele mining
RNAseq
Lycopersicum spp.
eIF4E
Tobacco etch virus
Origine
Importé de halUnités de recherche