Afficher la notice abrégée

Predicting the minimal translation apparatus: lessons from the reductive evolution of mollicutes

hal.structure.identifierUniversité Paris-Sud - Paris 11 [UP11]
dc.contributor.authorGROSJEAN, Henri
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBRETON, Marc
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIRAND-PUGNET, Pascal
hal.structure.identifierAgence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
dc.contributor.authorTARDY, Florence
hal.structure.identifierContrôle des maladies animales exotiques et émergentes [UMR CMAEE]
dc.contributor.authorTHIAUCOURT, François
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorCITTI, Christine
hal.structure.identifierUniversité Sciences et Technologies - Bordeaux 1 [UB]
dc.contributor.authorBARRÉ, Aurélien
hal.structure.identifierUniversité Paris-Sud - Paris 11 [UP11]
dc.contributor.authorYOSHIZAWA, Satoko
hal.structure.identifierUniversité Paris-Sud - Paris 11 [UP11]
dc.contributor.authorFOURMY, Dominique
hal.structure.identifierDepartment of Microbiology and Cell Science
dc.contributor.authorDE CRECY-LAGARD, Valerie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBLANCHARD, Alain
dc.date.issued2014
dc.identifier.issn1553-7390
dc.description.abstractEnMollicutes is a class of parasitic bacteria that have evolved from a common Firmicutes ancestor mostly by massive genome reduction. With genomes under 1 Mbp in size, most Mollicutes species retain the capacity to replicate and grow autonomously. The major goal of this work was to identify the minimal set of proteins that can sustain ribosome biogenesis and translation of the genetic code in these bacteria. Using the experimentally validated genes from the model bacteria Escherichia coli and Bacillus subtilis as input, genes encoding proteins of the core translation machinery were predicted in 39 distinct Mollicutes species, 33 of which are culturable. The set of 260 input genes encodes proteins involved in ribosome biogenesis, tRNA maturation and aminoacylation, as well as proteins cofactors required for mRNA translation and RNA decay. A core set of 104 of these proteins is found in all species analyzed. Genes encoding proteins involved in post-translational modifications of ribosomal proteins and translation cofactors, post-transcriptional modifications of t+rRNA, in ribosome assembly and RNA degradation are the most frequently lost. As expected, genes coding for aminoacyl-tRNA synthetases, ribosomal proteins and initiation, elongation and termination factors are the most persistent (i.e. conserved in a majority of genomes). Enzymes introducing nucleotides modifications in the anticodon loop of tRNA, in helix 44 of 16S rRNA and in helices 69 and 80 of 23S rRNA, all essential for decoding and facilitating peptidyl transfer, are maintained in all species. Reconstruction of genome evolution in Mollicutes revealed that, beside many gene losses, occasional gains by horizontal gene transfer also occurred. This analysis not only showed that slightly different solutions for preserving a functional, albeit minimal, protein synthetizing machinery have emerged in these successive rounds of reductive evolution but also has broad implications in guiding the reconstruction of a minimal cell by synthetic biology approaches.
dc.language.isoen
dc.publisherPublic Library of Science
dc.title.enPredicting the minimal translation apparatus: lessons from the reductive evolution of mollicutes
dc.typeArticle de revue
dc.identifier.doi10.1371/journal.pgen.1004363
dc.subject.halSciences du Vivant [q-bio]/Autre [q-bio.OT]
bordeaux.journalPLoS Genetics
bordeaux.pagee1004363
bordeaux.volume10
bordeaux.issue5
bordeaux.peerReviewedoui
hal.identifierhal-02639893
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02639893v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS%20Genetics&rft.date=2014&rft.volume=10&rft.issue=5&rft.spage=e1004363&rft.epage=e1004363&rft.eissn=1553-7390&rft.issn=1553-7390&rft.au=GROSJEAN,%20Henri&BRETON,%20Marc&SIRAND-PUGNET,%20Pascal&TARDY,%20Florence&THIAUCOURT,%20Fran%C3%A7ois&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée