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A protein kinase binds the C-terminal domain of the readthrough protein of Turnip yellows virus and regulates virus accumulation

hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorRODRIGUEZ-MEDINA, Caren
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorBOISSINOT, Sylvaine
hal.structure.identifierInstitut de Biologie Moléculaire des Plantes [IBMP]
dc.contributor.authorCHAPUIS, Sophie
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorGEREIGE, Dalya
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorRASTEGAR, Maryam
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorERDINGER, Monique
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorREVERS, Frederic
hal.structure.identifierInstitut de Biologie Moléculaire des Plantes [IBMP]
dc.contributor.authorZIEGLER-GRAFF, Véronique
hal.structure.identifierSanté de la vigne et qualité du vin [SVQV]
dc.contributor.authorBRAULT, Véronique
dc.date.issued2015
dc.identifier.issn0042-6822
dc.description.abstractEnTurnip yellows virus (TuYV), a phloem-limited virus, encodes a 74 kDa protein known as the readthrough protein (RT) involved in virus movement. We show here that a TuYV mutant deleted of the C-terminal part of the RT protein (TuYV-ΔRTCter) was affected in long-distance trafficking in a host-specific manner. By using the C-terminal domain of the RT protein as a bait in a yeast two-hybrid screen of a phloem cDNA library from Arabidopsis thaliana we identified the calcineurin B-like protein-interacting protein kinase-7 (AtCIPK7). Transient expression of a GFP:CIPK7 fusion protein in virus-inoculated Nicotiana benthamiana leaves led to local increase of wild-type TuYV accumulation, but not that of TuYV-ΔRTCter. Surprisingly, elevated virus titer in inoculated leaves did not result in higher TuYV accumulation in systemic leaves, which indicates that virus long-distance movement was not affected. Since GFP:CIPK7 was localized in or near plasmodesmata, CIPK7 could negatively regulate TuYV export from infected cells.
dc.language.isoen
dc.publisherElsevier
dc.subjectPlant;
dc.subject.enPolerovirus;
dc.subject.enPhloem;
dc.subject.enMovement;
dc.subject.enKinase;
dc.subject.enCIPK
dc.title.enA protein kinase binds the C-terminal domain of the readthrough protein of Turnip yellows virus and regulates virus accumulation
dc.typeArticle de revue
dc.identifier.doi10.1016/j.virol.2015.08.031
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalVirology
bordeaux.page44-53
bordeaux.volume486
bordeaux.peerReviewedoui
hal.identifierhal-02640267
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02640267v1
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