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Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism.

hal.structure.identifierUniversidade de São Paulo = University of São Paulo [USP]
hal.structure.identifierInstituto Nacional de Tecnología Agropecuaria [INTA]
dc.contributor.authorBERMÚDEZ, Luisa
hal.structure.identifierUniversidade de São Paulo = University of São Paulo [USP]
dc.contributor.authorDE GODOY, Fabiana
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALDET, Pierre
hal.structure.identifierUniversidade de São Paulo = University of São Paulo [USP]
dc.contributor.authorDEMARCO, Diego
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorOSORIO, Sonia
hal.structure.identifierInstituto Nacional de Tecnología Agropecuaria [INTA]
hal.structure.identifierConsejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] [CONICET]
dc.contributor.authorQUADRANA, Leandro
hal.structure.identifierUniversidade de São Paulo = University of São Paulo [USP]
dc.contributor.authorALMEIDA, Juliana
hal.structure.identifierConsejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] [CONICET]
hal.structure.identifierUniversidad de Córdoba = University of Córdoba [Córdoba]
dc.contributor.authorASIS, Ramón
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorFERNIE, Alisdair R
hal.structure.identifierUniversidade de São Paulo = University of São Paulo [USP]
dc.contributor.authorROSSI, Magdalena
hal.structure.identifierInstituto Nacional de Tecnología Agropecuaria [INTA]
hal.structure.identifierConsejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] [CONICET]
dc.contributor.authorCARRARI, Fernando
dc.date.issued2014
dc.identifier.issn0960-7412
dc.description.abstractEnLimitations in our understanding about the mechanisms that underlie source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive approach, this work reports the functional characterization of a DnaJ chaperone related-protein (named as SPA; sugar partition-affecting) that is involved in assimilate partitioning in tomato plants. SPA protein was found to be targeted to the chloroplast thylakoid membranes. SPA-RNAi tomato plants produced more and heavier fruits compared with controls, thus resulting in a considerable increment in harvest index. The transgenic plants also displayed increased pigment levels and reduced sucrose, glucose and fructose contents in leaves. Detailed metabolic and enzymatic activities analyses showed that sugar phosphate intermediates were increased while the activity of phosphoglucomutase, sugar kinases and invertases was reduced in the photosynthetic organs of the silenced plants. These changes would be anticipated to promote carbon export from foliar tissues. The combined results suggested that the tomato SPA protein plays an important role in plastid metabolism and mediates the source-sink relationships by affecting the rate of carbon translocation to fruits.
dc.language.isoen
dc.publisherWiley
dc.subjectfruit metabolism
dc.subject.enchloroplast metabolism
dc.subject.ensmall plastidial protein
dc.subject.ensource-sink partitioning
dc.subject.entomato
dc.title.enSilencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism.
dc.typeArticle de revue
dc.identifier.doi10.1111/tpj.12418
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPlant Journal
bordeaux.page676-87
bordeaux.volume77
bordeaux.issue5
bordeaux.peerReviewedoui
hal.identifierhal-02640583
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02640583v1
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