Silencing of the tomato Sugar Partitioning Affecting protein (SPA) modifies sink strength through a shift in leaf sugar metabolism.
BERMÚDEZ, Luisa
Universidade de São Paulo = University of São Paulo [USP]
Instituto Nacional de Tecnología Agropecuaria [INTA]
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Universidade de São Paulo = University of São Paulo [USP]
Instituto Nacional de Tecnología Agropecuaria [INTA]
BERMÚDEZ, Luisa
Universidade de São Paulo = University of São Paulo [USP]
Instituto Nacional de Tecnología Agropecuaria [INTA]
Universidade de São Paulo = University of São Paulo [USP]
Instituto Nacional de Tecnología Agropecuaria [INTA]
QUADRANA, Leandro
Instituto Nacional de Tecnología Agropecuaria [INTA]
Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] [CONICET]
Instituto Nacional de Tecnología Agropecuaria [INTA]
Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] [CONICET]
ASIS, Ramón
Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] [CONICET]
Universidad de Córdoba = University of Córdoba [Córdoba]
Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] [CONICET]
Universidad de Córdoba = University of Córdoba [Córdoba]
CARRARI, Fernando
Instituto Nacional de Tecnología Agropecuaria [INTA]
Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] [CONICET]
< Leer menos
Instituto Nacional de Tecnología Agropecuaria [INTA]
Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] [CONICET]
Idioma
en
Article de revue
Este ítem está publicado en
Plant Journal. 2014, vol. 77, n° 5, p. 676-87
Wiley
Resumen en inglés
Limitations in our understanding about the mechanisms that underlie source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive ...Leer más >
Limitations in our understanding about the mechanisms that underlie source-sink assimilate partitioning are increasingly becoming a major hurdle for crop yield enhancement via metabolic engineering. By means of a comprehensive approach, this work reports the functional characterization of a DnaJ chaperone related-protein (named as SPA; sugar partition-affecting) that is involved in assimilate partitioning in tomato plants. SPA protein was found to be targeted to the chloroplast thylakoid membranes. SPA-RNAi tomato plants produced more and heavier fruits compared with controls, thus resulting in a considerable increment in harvest index. The transgenic plants also displayed increased pigment levels and reduced sucrose, glucose and fructose contents in leaves. Detailed metabolic and enzymatic activities analyses showed that sugar phosphate intermediates were increased while the activity of phosphoglucomutase, sugar kinases and invertases was reduced in the photosynthetic organs of the silenced plants. These changes would be anticipated to promote carbon export from foliar tissues. The combined results suggested that the tomato SPA protein plays an important role in plastid metabolism and mediates the source-sink relationships by affecting the rate of carbon translocation to fruits.< Leer menos
Palabras clave
fruit metabolism
Palabras clave en inglés
chloroplast metabolism
small plastidial protein
source-sink partitioning
tomato
Orígen
Importado de HalCentros de investigación