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hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorOUIBRAHIM, Laurence
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorMAZIER, Marianne
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorESTEVAN, Joan
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPAGNY, Gaelle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDECROOCQ, Véronique
hal.structure.identifierUnité de Pathologie Végétale [PV]
dc.contributor.authorDESBIEZ, Cecile
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorMORETTI, André
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorGALLOIS, Jean-Luc
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorCARANTA, Carole
dc.date.issued2014
dc.identifier.issn0960-7412
dc.description.abstractEn<em>Arabidopsis thaliana</em> represents a valuable and efficient model to understand mechanisms underlying plant susceptibility to viral diseases. Here, we describe the identification and molecular cloning of a new gene responsible for recessive resistance to several isolates of Watermelon mosaic virus (WMV, genus Potyvirus) in the Arabidopsis Cvi-0 accession. rwm1 acts at an early stage of infection by impairing viral accumulation in initially infected leaf tissues. Map-based cloning delimited rwm1 on chromosome 1 in a 114-kb region containing 30 annotated genes. Positional and functional candidate gene analysis suggested that rwm1 encodes cPGK2 (At1g56190), an evolutionary conserved nucleus-encoded chloroplast phosphoglycerate kinase with a key role in cell metabolism. Comparative sequence analysis indicates that a single amino acid substitution (S78G) in the N-terminal domain of cPGK2 is involved in rwm1-mediated resistance. This mutation may have functional consequences because it targets a highly conserved residue, affects a putative phosphorylation site and occurs within a predicted nuclear localization signal. Transgenic complementation in Arabidopsis together with virus-induced gene silencing in<em> Nicotiana benthamiana</em> confirmed that cPGK2 corresponds to rwm1 and that the protein is required for efficient WMV infection. This work uncovers new insight into natural plant resistance mechanisms that may provide interesting opportunities for the genetic control of plant virus diseases. This article is protected by copyright. All rights reserved.
dc.language.isoen
dc.publisherWiley
dc.rights.urihttp://creativecommons.org/licenses/by-sa/
dc.subject.enPhosphoglycerate kinase
dc.subject.enPotyvirus
dc.subject.enWatermelon mosaic virus
dc.subject.enresistance gene
dc.subject.enArabidopsis
dc.title.enCloning of the <em>Arabidopsis</em> rwm1 gene for resistance to Watermelon mosaic virus points to a new function for natural virus resistance genes
dc.typeArticle de revue
dc.identifier.doi10.1111/tpj.12586
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
bordeaux.journalPlant Journal
bordeaux.page705-716
bordeaux.volume79
bordeaux.issue5
bordeaux.peerReviewedoui
hal.identifierhal-02640977
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02640977v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Plant%20Journal&amp;rft.date=2014&amp;rft.volume=79&amp;rft.issue=5&amp;rft.spage=705-716&amp;rft.epage=705-716&amp;rft.eissn=0960-7412&amp;rft.issn=0960-7412&amp;rft.au=OUIBRAHIM,%20Laurence&amp;MAZIER,%20Marianne&amp;ESTEVAN,%20Joan&amp;PAGNY,%20Gaelle&amp;DECROOCQ,%20V%C3%A9ronique&amp;rft.genre=article


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