Afficher la notice abrégée

Detection of 'Candidatus Phytoplasma brasiliense' in a new geographic region and existence of two genetically distinct dnaK genotypes

hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierUniversité Sciences et Technologies - Bordeaux 1 [UB]
hal.structure.identifiernational academy of science
dc.contributor.authorBALAKISHIYEVA, Gulnara
hal.structure.identifierInstitute of Horticulture and subtropical crops
dc.contributor.authorQURBANOV, Madat
hal.structure.identifiernational academy of science
dc.contributor.authorMAMMADOV, Alamdar
hal.structure.identifiernational academy of science
dc.contributor.authorBAYRAMOV, Shahniyar
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierUniversité Sciences et Technologies - Bordeaux 1 [UB]
dc.contributor.authorFOISSAC, Xavier
dc.date.issued2011
dc.identifier.issn1721-8861
dc.description.abstractEnIn September 2007, a peach tree (Prunus persica) displaying yellowing symptoms indicative of phytoplasma infection was sampled in Quba region of Azerbaijan. A phytoplasma was detected in the diseased peach tree by nested PCR amplification of its 16Sr DNA with universal primers for phytoplasmas. Phylogenetic analyses of the amplified 16S rDNA showed that the phytoplasma infecting peach tree corresponded to 'Candidatus Phytoplasma brasiliense', a phytoplasma not previously reported in the Euro-Mediterranean area. To set up a detection assay, cloning of a 'Ca. P. brasiliense' DNA fragment was undertaken by comparative RAPD. The amplified dnaK-dnaJ genetic locus was used to design a nested PCR assay able to amplify all 'Ca. P. brasiliense' strains of the subgroup 16SrXV-A without amplifying the related members of the group 16SrII. The use of this assay also confirmed detection for the first time of 'Ca. P. brasiliense' in diseased basil collected in south Lebanon.
dc.language.isoen
dc.publisherDepartment of Agricultural and Food Sciences
dc.subject.en'Ca. P. brasiliense'
dc.subject.enhibiscus witches' broom disease
dc.subject.endnaK gene
dc.subject.enmolecular characterization
dc.title.enDetection of 'Candidatus Phytoplasma brasiliense' in a new geographic region and existence of two genetically distinct dnaK genotypes
dc.typeArticle de revue
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalBulletin of Insectology
bordeaux.pageS61 - S62
bordeaux.volume64
bordeaux.peerReviewedoui
hal.identifierhal-02642356
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02642356v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Bulletin%20of%20Insectology&rft.date=2011&rft.volume=64&rft.spage=S61%20-%20S62&rft.epage=S61%20-%20S62&rft.eissn=1721-8861&rft.issn=1721-8861&rft.au=BALAKISHIYEVA,%20Gulnara&QURBANOV,%20Madat&MAMMADOV,%20Alamdar&BAYRAMOV,%20Shahniyar&FOISSAC,%20Xavier&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée