Detection of 'Candidatus Phytoplasma brasiliense' in a new geographic region and existence of two genetically distinct dnaK genotypes
BALAKISHIYEVA, Gulnara
Biologie du fruit et pathologie [BFP]
Université Sciences et Technologies - Bordeaux 1 [UB]
national academy of science
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Biologie du fruit et pathologie [BFP]
Université Sciences et Technologies - Bordeaux 1 [UB]
national academy of science
BALAKISHIYEVA, Gulnara
Biologie du fruit et pathologie [BFP]
Université Sciences et Technologies - Bordeaux 1 [UB]
national academy of science
Biologie du fruit et pathologie [BFP]
Université Sciences et Technologies - Bordeaux 1 [UB]
national academy of science
FOISSAC, Xavier
Biologie du fruit et pathologie [BFP]
Université Sciences et Technologies - Bordeaux 1 [UB]
< Leer menos
Biologie du fruit et pathologie [BFP]
Université Sciences et Technologies - Bordeaux 1 [UB]
Idioma
en
Article de revue
Este ítem está publicado en
Bulletin of Insectology. 2011, vol. 64, p. S61 - S62
Department of Agricultural and Food Sciences
Resumen en inglés
In September 2007, a peach tree (Prunus persica) displaying yellowing symptoms indicative of phytoplasma infection was sampled in Quba region of Azerbaijan. A phytoplasma was detected in the diseased peach tree by nested ...Leer más >
In September 2007, a peach tree (Prunus persica) displaying yellowing symptoms indicative of phytoplasma infection was sampled in Quba region of Azerbaijan. A phytoplasma was detected in the diseased peach tree by nested PCR amplification of its 16Sr DNA with universal primers for phytoplasmas. Phylogenetic analyses of the amplified 16S rDNA showed that the phytoplasma infecting peach tree corresponded to 'Candidatus Phytoplasma brasiliense', a phytoplasma not previously reported in the Euro-Mediterranean area. To set up a detection assay, cloning of a 'Ca. P. brasiliense' DNA fragment was undertaken by comparative RAPD. The amplified dnaK-dnaJ genetic locus was used to design a nested PCR assay able to amplify all 'Ca. P. brasiliense' strains of the subgroup 16SrXV-A without amplifying the related members of the group 16SrII. The use of this assay also confirmed detection for the first time of 'Ca. P. brasiliense' in diseased basil collected in south Lebanon.< Leer menos
Palabras clave en inglés
'Ca. P. brasiliense'
hibiscus witches' broom disease
dnaK gene
molecular characterization
Orígen
Importado de HalCentros de investigación