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Micro-Tom mutants for functional analysis of target genes and discovery of new alleles in tomato

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJUST, Daniel
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGARCIA, Virginie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFERNANDEZ, Lucie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBRES, Cécile
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUXION, Jean-Philippe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPETIT, Johann
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJORLY, Joana
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorASSALI, Julien
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBOURNONVILLE, Celine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFERRAND, Carine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALDET, Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLEMAIRE-CHAMLEY, Martine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMORI, Kentaro
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorOKABE, Yoshihiro
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorARIZUMI, Tohru
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorEZURA, Hiroshi
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
dc.date.issued2013
dc.identifier.issn1342-4580
dc.description.abstractEnTomato is currently the model plant for fleshy fruit development and for Solanaceae species. Recent genomic approaches including transcriptome, proteome and metabolome analyses and genetic mapping have produced a wealth of candidate genes whose function needs to be assessed. The recent development in model and crop plants of TILLING (Targeting Induced Local Lesions IN Genomes), which reveals allelic series corresponding to several independent point mutations, and the current availability of deep sequencing tools further increase the interest of generating artificially-induced genetic diversity in tomato. We describe here the generation and use of EMS (ethyl methanesulfonate) tomato mutants in the miniature cultivar Micro-Tom and provide as example the identification of new fruit size and morphology mutants. We further propose new deep sequencing-based strategies for the discovery of mutations underlying phenotypic variations observed in mutant collections that will considerably increase the interest of exploiting Micro-Tom mutant collections for gene discovery in tomato.
dc.language.isoen
dc.publisherJapanese Society for Plant Cell and Molecular Biology
dc.subjectmutant
dc.subjectpoint mutation
dc.subjectmodel system
dc.subject.endeep sequencing
dc.subject.enphenotype
dc.subject.endatabase
dc.subject.entomato
dc.subject.entilling
dc.subject.enforward genetic
dc.subject.enfruit development
dc.subject.encultivated tomato
dc.subject.enprovide insight
dc.subject.eninduced mutation
dc.subject.encuticle formation
dc.subject.enreverse genetic
dc.subject.encandidate gene
dc.subject.enarabidopsis
dc.title.enMicro-Tom mutants for functional analysis of target genes and discovery of new alleles in tomato
dc.typeArticle de revue
dc.identifier.doi10.5511/plantbiotechnology.13.0622a
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPlant Biotechnology
bordeaux.page225-231
bordeaux.volume30
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-02644308
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02644308v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20Biotechnology&rft.date=2013&rft.volume=30&rft.issue=3&rft.spage=225-231&rft.epage=225-231&rft.eissn=1342-4580&rft.issn=1342-4580&rft.au=JUST,%20Daniel&GARCIA,%20Virginie&FERNANDEZ,%20Lucie&BRES,%20C%C3%A9cile&MAUXION,%20Jean-Philippe&rft.genre=article


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