POORTER, Hendrik; FIORANI, Fabio; PLANCK, Max; SCHURR, Uli; FINCK, Alex; GIBON, Yves; USADEL, Björn; MUNNS, Rana; ATKIN, Owen K.; TARDIEU, Francois; PONS, Thijs L.

doi:10.1071/FP12028

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POORTER, Hendrik
Forschungszentrum Jülich GmbH | Centre de recherche de Jülich | Jülich Research Centre [FZJ]

FIORANI, Fabio
Forschungszentrum Jülich GmbH | Centre de recherche de Jülich | Jülich Research Centre [FZJ]

PLANCK, Max
Max Planck Institute of Molecular Plant Physiology [MPI-MP]

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Article de revue

Ce document a été publié dans

Functional Plant Biology. 2012, vol. 39, n° 10-11, p. 821-838

CSIRO Publishing

Résumé en anglais

Every year thousands of experiments are conducted using plants grown under more-or-less controlled environmental conditions. The aim of many such experiments is to compare the phenotype of different species or genotypes in a specific environment, or to study plant performance under a range of suboptimal conditions. Our paper aims to bring together the minimum knowledge necessary for a plant biologist to set up such experiments and apply the environmental conditions that are appropriate to answer the questions of interest. We first focus on the basic choices that have to be made with regard to the experimental setup (e.g. where are the plants grown; what rooting medium; what pot size). Second, we present practical considerations concerning the number of plants that have to be analysed considering the variability in plant material and the required precision. Third, we discuss eight of the most important environmental factors for plant growth (light quantity, light quality, CO2, nutrients, air humidity, water, temperature and salinity); what critical issues should be taken into account to ensure proper growth conditions in controlled environments and which specific aspects need attention if plants are challenged with a certain a-biotic stress factor. Finally, we propose a simple checklist that could be used for tracking and reporting experimental conditions.< Réduire

Mots clés

environmental conditions

Mots clés en anglais

controlled experiments

glasshouse

growth chamber

plant growth stress

DOI

http://dx.doi.org/10.1071/FP12028

Projet Européen

Drought-tolerant yielding plants

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332