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High temperature inhibits ascorbate recycling and light stimulation of the ascorbate pool in tomato despite increased expression of biosynthesis genes

hal.structure.identifierUnité de recherche Plantes et Systèmes de Culture Horticoles [PSH]
dc.contributor.authorMASSOT, Capucine
hal.structure.identifierUnité de recherche Plantes et Systèmes de Culture Horticoles [PSH]
dc.contributor.authorBANCEL, Doriane
hal.structure.identifierEA 4279 Laboratoire Physiologie des Fruits et Légumes
dc.contributor.authorLOPEZ LAURI, Félicie
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorTRUFFAULT, Vincent
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALDET, Pierre
hal.structure.identifierGénétique et Amélioration des Fruits et Légumes [GAFL]
dc.contributor.authorSTEVENS, Rebecca
hal.structure.identifierUnité de recherche Plantes et Systèmes de Culture Horticoles [PSH]
dc.contributor.authorGAUTIER, Hélène
dc.date.issued2013
dc.identifier.issn1932-6203
dc.description.abstractEnUnderstanding how the fruit microclimate affects ascorbate (AsA) biosynthesis, oxidation and recycling is a great challenge in improving fruit nutritional quality. For this purpose, tomatoes at breaker stage were harvested and placed in controlled environment conditions at different temperatures (12, 17, 23, 27 and 31 °C) and irradiance regimes (darkness or 150 µmol m(-2) s(-1)). Fruit pericarp tissue was used to assay ascorbate, glutathione, enzymes related to oxidative stress and the AsA/glutathione cycle and follow the expression of genes coding for 5 enzymes of the AsA biosynthesis pathway (GME, VTC2, GPP, L-GalDH, GLDH). The AsA pool size in pericarp tissue was significantly higher under light at temperatures below 27 °C. In addition, light promoted glutathione accumulation at low and high temperatures. At 12 °C, increased AsA content was correlated with the enhanced expression of all genes of the biosynthesis pathway studied, combined with higher DHAR and MDHAR activities and increased enzymatic activities related to oxidative stress (CAT and APX). In contrast, at 31 °C, MDHAR and GR activities were significantly reduced under light indicating that enzymes of the AsA/glutathione cycle may limit AsA recycling and pool size in fruit pericarp, despite enhanced expression of genes coding for AsA biosynthesis enzymes. In conclusion, this study confirms the important role of fruit microclimate in the regulation of fruit pericarp AsA content, as under oxidative conditions (12 °C, light) total fruit pericarp AsA content increased up to 71%. Moreover, it reveals that light and temperature interact to regulate both AsA biosynthesis gene expression in tomato fruits and AsA oxidation and recycling.
dc.language.isoen
dc.publisherPublic Library of Science
dc.title.enHigh temperature inhibits ascorbate recycling and light stimulation of the ascorbate pool in tomato despite increased expression of biosynthesis genes
dc.typeArticle de revue
dc.identifier.doi10.1371/journal.pone.0084474
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPLoS ONE
bordeaux.page13 p.
bordeaux.volume8
bordeaux.issue12
bordeaux.peerReviewedoui
hal.identifierhal-02645038
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02645038v1
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